X0508 4 2025-06-16 0001980845 enGene Holdings Inc. ENGN 0001787363 Pott Amy C/O 4868 RUE LEVY, SUITE 220 SAINT-LAURENT A8 H4R 2P1 QUEBEC, CANADA true Chief Global Comm. Ofc. false Stock Option (Right to Buy) 3.31 2025-06-16 4 A 0 400000 0 A 2035-06-16 Common Shares 400000 400000 D Non-qualified stock option grant awarded as an inducement award outside of the Amended and Restated enGene Holdings Inc. 2023 Incentive Equity Plan in accordance with NASDAQ Listing Rule 5635(c)(4). This option vests at 25% on May 27, 2026, which is the first anniversary of the employment commencement date, with the remaining portion to vest monthly over the remaining three years, subject to the reporting person's continued service. /s/ Lee Giguere, as attorney-in-fact for the Reporting Person 2025-06-16