<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001955607</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001842718</issuerCik>
      <issuerName>INTEGRAL AD SCIENCE HOLDING CORP.</issuerName>
      <secFileNumber>001-40557</secFileNumber>
      <issuerAddress>
        <com:street1>99 WALL STREET</com:street1>
        <com:street2>#1950</com:street2>
        <com:city>NEW YORK</com:city>
        <com:stateOrCountry>NY</com:stateOrCountry>
        <com:zipCode>10005</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>(646) 278-4871</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>SECOR TANIA</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Officer</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Fidelity Brokerage Services LLC</name>
        <address>
          <com:street1>900 Salem Street</com:street1>
          <com:city>Smithfield</com:city>
          <com:stateOrCountry>RI</com:stateOrCountry>
          <com:zipCode>02917</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>7889</noOfUnitsSold>
      <aggregateMarketValue>87384.09</aggregateMarketValue>
      <noOfUnitsOutstanding>161364929</noOfUnitsOutstanding>
      <approxSaleDate>09/09/2024</approxSaleDate>
      <securitiesExchangeName>NASDAQ</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>09/05/2024</acquiredDate>
      <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>7889</amountOfSecuritiesAcquired>
      <paymentDate>09/05/2024</paymentDate>
      <natureOfPayment>Compensation</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Tania R. Secor</name>
        <address>
          <com:street1>12 E 49th Street</com:street1>
          <com:street2>20th Floor</com:street2>
          <com:city>New York</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10017</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/10/2024</saleDate>
      <amountOfSecuritiesSold>6382</amountOfSecuritiesSold>
      <grossProceeds>57553.51</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Tania R. Secor</name>
        <address>
          <com:street1>12 E 49th Street</com:street1>
          <com:street2>20th Floor</com:street2>
          <com:city>New York</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10017</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>07/08/2024</saleDate>
      <amountOfSecuritiesSold>4564</amountOfSecuritiesSold>
      <grossProceeds>44159.44</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Tania R. Secor</name>
        <address>
          <com:street1>12 E 49th Street</com:street1>
          <com:street2>20th Floor</com:street2>
          <com:city>New York</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10017</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>09/06/2024</saleDate>
      <amountOfSecuritiesSold>6643</amountOfSecuritiesSold>
      <grossProceeds>74691.90</grossProceeds>
    </securitiesSoldInPast3Months>
    <remarks>Sale includes an amount necessary to cover a tax obligation resulting from the settlement of a vested equity award distribution.</remarks>
    <noticeSignature>
      <noticeDate>09/09/2024</noticeDate>
      <signature>/s/ Gerardo Ibarra-Munoz, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Tania R. Secor</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
