<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001954493</cik>
        <entityName>Pelion Ventures VIII, L.P.</entityName>
        <issuerAddress>
            <street1>2750 E. COTTONWOOD PARKWAY, SUITE 600</street1>
            <city>SALT LAKE CITY</city>
            <stateOrCountry>UT</stateOrCountry>
            <stateOrCountryDescription>UTAH</stateOrCountryDescription>
            <zipCode>84121</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>801-365-0262</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Limited Partnership</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2022</value>
        </yearOfInc>
    </primaryIssuer>

    <issuerList>
        <issuer>
            <cik>0001954492</cik>
            <entityName>Pelion Ventures VIII-A, L.P.</entityName>
            <issuerAddress>
                <street1>2750 E. COTTONWOOD PARKWAY, SUITE 600</street1>
                <city>SALT LAKE CITY</city>
                <stateOrCountry>UT</stateOrCountry>
                <stateOrCountryDescription>UTAH</stateOrCountryDescription>
                <zipCode>84121</zipCode>
            </issuerAddress>
            <issuerPhoneNumber>801-365-0262</issuerPhoneNumber>
            <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
            <issuerPreviousNameList>
                <value>None</value>
            </issuerPreviousNameList>
            <edgarPreviousNameList>
                <value>None</value>
            </edgarPreviousNameList>
            <entityType>Limited Partnership</entityType>
            <yearOfInc>
                <withinFiveYears>true</withinFiveYears>
                <value>2022</value>
            </yearOfInc>
        </issuer>
    </issuerList>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>N/A</firstName>
                <lastName>Pelion Venture Partners VIII, L.L.C.</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>2750 E. Cottonwood Parkway, Suite 600</street1>
                <city>Salt Lake City</city>
                <stateOrCountry>UT</stateOrCountry>
                <stateOrCountryDescription>UTAH</stateOrCountryDescription>
                <zipCode>84121</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>The General Partner of Pelion Ventures VIII, L.P. (&quot;Fund VIII&quot;) and Pelion Ventures VIII-A, L.P. (&quot;Fund VIII-A&quot; and, together with Fund VIII, the &quot;Issuers&quot;)</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Blake</firstName>
                <middleName>G.</middleName>
                <lastName>Modersitzki</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>2750 E. Cottonwood Parkway, Suite 600</street1>
                <city>Salt Lake City</city>
                <stateOrCountry>UT</stateOrCountry>
                <stateOrCountryDescription>UTAH</stateOrCountryDescription>
                <zipCode>84121</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Manager of the General Partner of the Issuer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Chris</firstName>
                <lastName>Cooper</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>2750 E. Cottonwood Parkway, Suite 600</street1>
                <city>Salt Lake City</city>
                <stateOrCountry>UT</stateOrCountry>
                <stateOrCountryDescription>UTAH</stateOrCountryDescription>
                <zipCode>84121</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Manager of the General Partner of the Issuer</relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pooled Investment Fund</industryGroupType>
            <investmentFundInfo>
                <investmentFundType>Venture Capital Fund</investmentFundType>
                <is40Act>false</is40Act>
            </investmentFundInfo>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
            <item>3C</item>
            <item>3C.1</item>
            <item>3C.7</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001954493-23-000002</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2023-04-25</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isPooledInvestmentFundType>true</isPooledInvestmentFundType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Greenstone Placement Management FZ-LLC</recipientName>
                <recipientCRDNumber>None</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>Boulevard Plaza Tower II, 27th Floor</street1>
                    <city>Dubai</city>
                    <stateOrCountry>C0</stateOrCountry>
                    <stateOrCountryDescription>UNITED ARAB EMIRATES</stateOrCountryDescription>
                    <zipCode>337-1500</zipCode>
                </recipientAddress>
                <statesOfSolicitationList></statesOfSolicitationList>
                <foreignSolicitation>true</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>250000000</totalOfferingAmount>
            <totalAmountSold>145850000</totalAmountSold>
            <totalRemaining>104150000</totalRemaining>
            <clarificationOfResponse>Amount disclosed in Item 13(b) includes $26,000,000 in commitments associated with a concurrent offering by the Issuers under Regulation S</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>22</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>260000</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse>A placement agent has been retained and will receive a fee to be determined based on the amount of certain commitments received by the Issuers.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse>The General Partner of the Issuers (or an affiliate thereof) is entitled to a management fee.</clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>Pelion Ventures VIII, L.P.</issuerName>
                <signatureName>/s/Blake G. Modersitzki</signatureName>
                <nameOfSigner>Blake G. Modersitzki</nameOfSigner>
                <signatureTitle>Manager of the General Partner of the Issuer</signatureTitle>
                <signatureDate>2023-05-31</signatureDate>
            </signature>
            <signature>
                <issuerName>Pelion Ventures VIII-A, L.P.</issuerName>
                <signatureName>/s/Blake G. Modersitzki</signatureName>
                <nameOfSigner>Blake G. Modersitzki</nameOfSigner>
                <signatureTitle>Manager of the General Partner of the Issuer</signatureTitle>
                <signatureDate>2023-05-31</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
