<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/schedule13g" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>SCHEDULE 13G</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001896206</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <coverPageHeader>
      <securitiesClassTitle>Common Stock, par value $0.01 per share</securitiesClassTitle>
      <eventDateRequiresFilingThisStatement>02/27/2026</eventDateRequiresFilingThisStatement>
      <issuerInfo>
        <issuerCik>0001951067</issuerCik>
        <issuerName>C3is Inc.</issuerName>
        <issuerCusip>Y18284177</issuerCusip>
        <issuerPrincipalExecutiveOfficeAddress>
          <com:street1>331 KIFISSIAS AVENUE</com:street1>
          <com:street2>ERITHREA</com:street2>
          <com:city>ATHENS</com:city>
          <com:stateOrCountry>J3</com:stateOrCountry>
          <com:zipCode>14561</com:zipCode>
        </issuerPrincipalExecutiveOfficeAddress>
      </issuerInfo>
      <designateRulesPursuantThisScheduleFiled>
        <designateRulePursuantThisScheduleFiled>Rule 13d-1(c)</designateRulePursuantThisScheduleFiled>
      </designateRulesPursuantThisScheduleFiled>
    </coverPageHeader>
    <coverPageHeaderReportingPersonDetails>

      <reportingPersonName>Alta Partners LLC</reportingPersonName>
      <citizenshipOrOrganization>NY</citizenshipOrOrganization>
      <reportingPersonBeneficiallyOwnedNumberOfShares>
        <soleVotingPower>56176.00</soleVotingPower>
        <sharedVotingPower>0.00</sharedVotingPower>
        <soleDispositivePower>56176.00</soleDispositivePower>
        <sharedDispositivePower>0.00</sharedDispositivePower>
      </reportingPersonBeneficiallyOwnedNumberOfShares>
      <reportingPersonBeneficiallyOwnedAggregateNumberOfShares>56176.00</reportingPersonBeneficiallyOwnedAggregateNumberOfShares>
      <classPercent>5.2</classPercent>
      <typeOfReportingPerson>OO</typeOfReportingPerson>
    </coverPageHeaderReportingPersonDetails>
    <items>
      <item1>
        <issuerName>C3is Inc.</issuerName>
        <issuerPrincipalExecutiveOfficeAddress>331 KIFISSIAS AVENUE, ERITHREA, ATHENS, GREECE, 14561</issuerPrincipalExecutiveOfficeAddress>
      </item1>
      <item2>
        <filingPersonName>Alta Partners LLC</filingPersonName>
        <principalBusinessOfficeOrResidenceAddress>1205 Franklin Avenue
Garden City, New York 11530</principalBusinessOfficeOrResidenceAddress>
        <citizenship>New York Limited Liability Company</citizenship>
      </item2>
      <item3>
        <notApplicableFlag>Y</notApplicableFlag>
      </item3>
      <item4>
        <amountBeneficiallyOwned>56,176 shares, representing shares issuable upon exercise of warrants. </amountBeneficiallyOwned>
        <classPercent>5.2%</classPercent>
        <numberOfSharesPersonHas>
          <solePowerOrDirectToVote>56,176 shares</solePowerOrDirectToVote>
          <sharedPowerOrDirectToVote>0 shares</sharedPowerOrDirectToVote>
          <solePowerOrDirectToDispose>56,176 shares</solePowerOrDirectToDispose>
          <sharedPowerOrDirectToDispose>0 shares</sharedPowerOrDirectToDispose>
        </numberOfSharesPersonHas>
      </item4>
      <item5>
        <notApplicableFlag>Y</notApplicableFlag>
        <classOwnership5PercentOrLess>N</classOwnership5PercentOrLess>
      </item5>
      <item6>
        <notApplicableFlag>Y</notApplicableFlag>
      </item6>
      <item7>
        <notApplicableFlag>Y</notApplicableFlag>
      </item7>
      <item8>
        <notApplicableFlag>Y</notApplicableFlag>
      </item8>
      <item9>
        <notApplicableFlag>Y</notApplicableFlag>
      </item9>
      <item10>
        <notApplicableFlag>N</notApplicableFlag>
        <certifications>By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under &amp;#167; 240.14a-11.

By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under &amp;#167; 240.14a-11.

</certifications>
      </item10>
    </items>
    <signatureInformation>
      <reportingPersonName>Alta Partners LLC</reportingPersonName>
      <signatureDetails>
        <signature>/s/ Steven Cohen</signature>
        <title>Steven Cohen, Managing Member</title>
        <date>02/27/2026</date>
      </signatureDetails>
    </signatureInformation>
  </formData>
</edgarSubmission>
