X0508 4 2024-03-31 0 0001870144 Cardio Diagnostics Holdings, Inc. CDIO 0001950394 Intrater James C/O CARDIO DIAGNOSTICS HOLDINGS INC. 311 W. SUPERIOR ST. SUITE 444 CHICAGO IL 60654 1 0 0 0 0 Common Stock 2024-03-31 4 M 0 8803 1.42 A 71596 D Restricted Stock Units 0 2024-03-31 4 M 0 12500 0 A 2024-03-31 2024-12-31 Common Stock 12500 37500 D Represents the settlement of restricted stock units (RSUs) that vested on March 31, 2024 through the issuance of shares of Common Stock. Each RSU is the economic equivalent of one share of Cardio Diagnostics Holdings, Inc. common stock. On March 31, 2024, $12,500 in value of RSUs vested and were settled at the price of $1.42, which was the closing price of the Company Common Stock on the vesting date. The settlement shares are reflected in Table I. On January 23, 2024, the reporting person was awarded $50,000 in value of RSUs, $12,500 in value of which vested on March 31, 2024. Subject to continued service with the Issuer on the vesting date, the RSUs will continue to vest $12,500 in value on June 30, 2024, $12,500 in value on September30, 2024 and $12,500 in value on December 31, 2024, based on the closing price of the Issuers common stock on the applicable vesting date. The RSUs were granted pursuant to the Issuers 2022 Equity Incentive Plan. /s/ Elisa Luqman as attorney-in-fact for James Intrater 2024-04-08