EXHIBIT 1

JOINT FILING AGREEMENT

The undersigned hereby agree that the statement on Schedule 13G with respect to the Common Stock of Alto Neuroscience, Inc., dated as of May 15, 2025, is, and any amendments thereto (including amendments on Schedule 13D) signed by each of the undersigned shall be, filed on behalf of each of us pursuant to and in accordance with the provisions of Rule 13d-1(k) under the Securities Exchange Act of 1934, as amended.

Dated: May 15, 2025
	K2 HEALTHVENTURES EQUITY TRUST LLC
	By:	/s/ Anup Arora
	Name:	Anup Arora
	Title:	Managing Member
	PARAG SHAH
	By:	/s/ Parag Shah
	ANUP ARORA
	By:	/s/ Anup Arora