<?xml version="1.0" encoding="UTF-8"?><own:edgarSubmission xmlns:own="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <!-- Generated by Workiva -->
  <own:headerData>
    <own:submissionType>144</own:submissionType>
    <own:filerInfo>
      <own:filer>
        <own:filerCredentials>
          <own:cik>0001405290</own:cik>
          <own:ccc>XXXXXXXX</own:ccc>
        </own:filerCredentials>
      </own:filer>
      <own:liveTestFlag>LIVE</own:liveTestFlag>



    </own:filerInfo>
  </own:headerData>
  <own:formData>
    <own:issuerInfo>
      <own:issuerCik>0001937993</own:issuerCik>
      <own:issuerName>CADRENAL THERAPEUTICS, INC.</own:issuerName>
      <own:issuerAddress>
        <com:street1>822 A1A NORTH</com:street1>
        <com:street2>SUITE 306</com:street2>
        <com:city>PONTE VEDRA BEACH</com:city>
        <com:stateOrCountry>FL</com:stateOrCountry>
        <com:zipCode>32082</com:zipCode>
      </own:issuerAddress>
      <own:issuerContactPhone>904-300-0701</own:issuerContactPhone>
      <own:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Quang X. Pham</own:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <own:relationshipsToIssuer>
        <own:relationshipToIssuer>Officer</own:relationshipToIssuer>
      </own:relationshipsToIssuer>
    </own:issuerInfo>
    <own:securitiesInformation>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:brokerOrMarketmakerDetails>
        <own:name>Merrill Lynch</own:name>
        <own:address>
          <com:street1>824 A1A North</com:street1>
          <com:street2>Suite 200</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:brokerOrMarketmakerDetails>
      <own:noOfUnitsSold>40000</own:noOfUnitsSold>
      <own:aggregateMarketValue>148400</own:aggregateMarketValue>
      <own:noOfUnitsOutstanding>2868247</own:noOfUnitsOutstanding>
      <own:approxSaleDate>06/15/2026</own:approxSaleDate>
      <own:securitiesExchangeName>NASDAQ</own:securitiesExchangeName>
    </own:securitiesInformation>
    <own:securitiesToBeSold>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:acquiredDate>01/25/2022</own:acquiredDate>
      <own:natureOfAcquisitionTransaction>Founders Shares</own:natureOfAcquisitionTransaction>
      <own:nameOfPersonfromWhomAcquired>Issuer</own:nameOfPersonfromWhomAcquired>
      <own:isGiftTransaction>N</own:isGiftTransaction>
      <own:amountOfSecuritiesAcquired>40000</own:amountOfSecuritiesAcquired>
      <own:paymentDate>01/25/2022</own:paymentDate>
      <own:natureOfPayment>N/A</own:natureOfPayment>
    </own:securitiesToBeSold>
    <own:nothingToReportFlagOnSecuritiesSoldInPast3Months>N</own:nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>05/11/2026</own:saleDate>
      <own:amountOfSecuritiesSold>6476</own:amountOfSecuritiesSold>
      <own:grossProceeds>37349.02</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>05/12/2026</own:saleDate>
      <own:amountOfSecuritiesSold>3298</own:amountOfSecuritiesSold>
      <own:grossProceeds>17428.92</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>05/22/2026</own:saleDate>
      <own:amountOfSecuritiesSold>1165</own:amountOfSecuritiesSold>
      <own:grossProceeds>6627.7</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>05/28/2026</own:saleDate>
      <own:amountOfSecuritiesSold>2989</own:amountOfSecuritiesSold>
      <own:grossProceeds>14739.78</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>05/29/2026</own:saleDate>
      <own:amountOfSecuritiesSold>303</own:amountOfSecuritiesSold>
      <own:grossProceeds>1518.7</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/02/2026</own:saleDate>
      <own:amountOfSecuritiesSold>349</own:amountOfSecuritiesSold>
      <own:grossProceeds>1555.82</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/03/2026</own:saleDate>
      <own:amountOfSecuritiesSold>2323</own:amountOfSecuritiesSold>
      <own:grossProceeds>9892.99</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/04/2026</own:saleDate>
      <own:amountOfSecuritiesSold>3898</own:amountOfSecuritiesSold>
      <own:grossProceeds>17206.23</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/05/2026</own:saleDate>
      <own:amountOfSecuritiesSold>2064</own:amountOfSecuritiesSold>
      <own:grossProceeds>9124.36</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/08/2026</own:saleDate>
      <own:amountOfSecuritiesSold>5893</own:amountOfSecuritiesSold>
      <own:grossProceeds>25815.88</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/09/2026</own:saleDate>
      <own:amountOfSecuritiesSold>12992</own:amountOfSecuritiesSold>
      <own:grossProceeds>53298.99</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/10/2026</own:saleDate>
      <own:amountOfSecuritiesSold>3500</own:amountOfSecuritiesSold>
      <own:grossProceeds>14717.5</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/11/2026</own:saleDate>
      <own:amountOfSecuritiesSold>1462</own:amountOfSecuritiesSold>
      <own:grossProceeds>6021.35</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:securitiesSoldInPast3Months>
      <own:sellerDetails>
        <own:name>Quang X. Pham</own:name>
        <own:address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </own:address>
      </own:sellerDetails>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:saleDate>06/12/2026</own:saleDate>
      <own:amountOfSecuritiesSold>12996</own:amountOfSecuritiesSold>
      <own:grossProceeds>51180.32</own:grossProceeds>
    </own:securitiesSoldInPast3Months>
    <own:remarks>Locking in the four-week average for week ending 6/12/26 for 332,006 shares.</own:remarks>
    <own:noticeSignature>
      <own:noticeDate>06/15/2026</own:noticeDate>
      <own:planAdoptionDates>
        <own:planAdoptionDate>09/23/2025</own:planAdoptionDate>
      </own:planAdoptionDates>
      <own:signature>Quang X. Pham</own:signature>
    </own:noticeSignature>
  </own:formData>
</own:edgarSubmission>
