<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns0="http://www.sec.gov/edgar/common">
<headerData>
<submissionType>144</submissionType>
<filerInfo>
<filer>
<filerCredentials>
<cik>0002012446</cik>
<ccc>XXXXXXXX</ccc>
</filerCredentials>
</filer>
<liveTestFlag>LIVE</liveTestFlag>
</filerInfo>
</headerData>
<formData>
<issuerInfo>
<issuerCik>0001921865</issuerCik>
<issuerName>ASP Isotopes Inc.</issuerName>
<secFileNumber>001-41555</secFileNumber>
<issuerAddress>
<ns0:street1>601 Pennsylvania Avenue NW</ns0:street1>
<ns0:street2>South Building, Suite 900</ns0:street2>
<ns0:city>Washington</ns0:city>
<ns0:stateOrCountry>DC</ns0:stateOrCountry>
<ns0:zipCode>20004</ns0:zipCode>
</issuerAddress>
<issuerContactPhone>2027562245</issuerContactPhone>
<nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Gorley Michael</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
<relationshipsToIssuer>
<relationshipToIssuer>DIRECTOR</relationshipToIssuer>
</relationshipsToIssuer>
</issuerInfo>
<securitiesInformation>
<securitiesClassTitle>Common Stock</securitiesClassTitle>
<brokerOrMarketmakerDetails>
<name>Independent Trading Group (ITG) Inc.</name>
<address>
<ns0:street1>33 Yonge Street</ns0:street1>
<ns0:street2>Suite 420</ns0:street2>
<ns0:city>Toronto</ns0:city>
<ns0:stateOrCountry>AK</ns0:stateOrCountry>
<ns0:zipCode>ON M5E 1G4</ns0:zipCode>
</address>
</brokerOrMarketmakerDetails>
<noOfUnitsSold>64000</noOfUnitsSold>
<aggregateMarketValue>662400</aggregateMarketValue>
<noOfUnitsOutstanding>91913109</noOfUnitsOutstanding>
<approxSaleDate>09/30/2025</approxSaleDate>
<securitiesExchangeName>NASDAQ</securitiesExchangeName>
</securitiesInformation>
<securitiesToBeSold>
<securitiesClassTitle>Common Stock</securitiesClassTitle>
<acquiredDate>10/23/2023</acquiredDate>
<natureOfAcquisitionTransaction>Acquired as compensation -- Restricted Stock Award</natureOfAcquisitionTransaction>
<nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
<isGiftTransaction>N</isGiftTransaction>
<amountOfSecuritiesAcquired>93458</amountOfSecuritiesAcquired>
<paymentDate>10/23/2023</paymentDate>
<natureOfPayment>NA</natureOfPayment>
</securitiesToBeSold>
<nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
<remarks>The sale of shares reported in this Form 144 includes an amount for the purpose of satisfying the Reporting Person's income tax liabilities resulting from the vesting of previously granted restricted stock awards pursuant to an issuer equity incentive plan.  The Aggregate Market Value of the shares to be sold is based on the closing market price of $10.35 on September 29, 2025.</remarks>
<noticeSignature>
<noticeDate>09/30/2025</noticeDate>
<planAdoptionDates/>
<signature>/s/ Donald Ainscow, as attorney-in-fact for Michael Gorley</signature>
</noticeSignature>
</formData>
</edgarSubmission>
