<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/schedule13g" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>SCHEDULE 13G</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <!-- Field: Pseudo-Tag; ID: Name; Data: Spieker Warren Edward JR -->
          <cik>0002080807</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>


    </filerInfo>
  </headerData>
  <formData>
    <coverPageHeader>
      <securitiesClassTitle>Common Stock, $0.0001 par value</securitiesClassTitle>
      <eventDateRequiresFilingThisStatement>07/23/2025</eventDateRequiresFilingThisStatement>
      <issuerInfo>
        <issuerCik>0001918080</issuerCik>
        <issuerName>DEEP ISOLATION NUCLEAR, INC.</issuerName>
        <issuerCusip>000000000</issuerCusip>
        <issuerPrincipalExecutiveOfficeAddress>
          <com:street1>2001 Addison, Suite 300,</com:street1>
          <com:city>Berkeley</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94704</com:zipCode>
        </issuerPrincipalExecutiveOfficeAddress>
      </issuerInfo>
      <designateRulesPursuantThisScheduleFiled>
        <designateRulePursuantThisScheduleFiled>Rule 13d-1(c)</designateRulePursuantThisScheduleFiled>
      </designateRulesPursuantThisScheduleFiled>
    </coverPageHeader>
    <coverPageHeaderReportingPersonDetails>


      <reportingPersonName>Warren E. Spieker, Jr., individually and as Trustee of the Spieker Living Trust UAD 3/12/02</reportingPersonName>
      <citizenshipOrOrganization>X1</citizenshipOrOrganization>
      <reportingPersonBeneficiallyOwnedNumberOfShares>
        <soleVotingPower>1291864.00</soleVotingPower>
        <sharedVotingPower>4611567.00</sharedVotingPower>
        <soleDispositivePower>1291864.00</soleDispositivePower>
        <sharedDispositivePower>4611567.00</sharedDispositivePower>
      </reportingPersonBeneficiallyOwnedNumberOfShares>
      <reportingPersonBeneficiallyOwnedAggregateNumberOfShares>5903431.00</reportingPersonBeneficiallyOwnedAggregateNumberOfShares>
      <aggregateAmountExcludesCertainSharesFlag>N</aggregateAmountExcludesCertainSharesFlag>
      <classPercent>10.3</classPercent>
      <typeOfReportingPerson>IN</typeOfReportingPerson>
      <comments>Based on 57,372,749 shares of common stock issued and outstanding as of July 23, 2025.</comments>
    </coverPageHeaderReportingPersonDetails>
    <items>
      <item1>
        <issuerName>DEEP ISOLATION NUCLEAR, INC.</issuerName>
        <issuerPrincipalExecutiveOfficeAddress>2001 Addison, Suite 300, Berkeley, CA 94704</issuerPrincipalExecutiveOfficeAddress>
      </item1>
      <item2>
        <filingPersonName>Warren E. Spieker, Jr.</filingPersonName>
        <principalBusinessOfficeOrResidenceAddress>707 Menlo Avenue Suite 100, Menlo Park, CA 94025</principalBusinessOfficeOrResidenceAddress>
        <citizenship>USA</citizenship>
      </item2>
      <item3>
        <notApplicableFlag>Y</notApplicableFlag>
      </item3>
      <item4>
        <amountBeneficiallyOwned>5,903,431</amountBeneficiallyOwned>
        <classPercent>10.3%</classPercent>
        <numberOfSharesPersonHas>
          <solePowerOrDirectToVote>1,291,864</solePowerOrDirectToVote>
          <sharedPowerOrDirectToVote>4,611,567</sharedPowerOrDirectToVote>
          <solePowerOrDirectToDispose>1,291,864</solePowerOrDirectToDispose>
          <sharedPowerOrDirectToDispose>4,611,567</sharedPowerOrDirectToDispose>
        </numberOfSharesPersonHas>
      </item4>
      <item5>
        <notApplicableFlag>Y</notApplicableFlag>
      </item5>
      <item6>
        <notApplicableFlag>N</notApplicableFlag>
        <ownershipMoreThan5PercentOnBehalfOfAnotherPerson>Securities reported in Item 4(c)(ii) and 4(c)(iv) on this Schedule 13G are owned directly by the Spieker Living Trust U/A/D 3/12/02 (the "Trust") and are beneficially owned by Warren E. Spieker, Jr. and Carol Spieker, as beneficiaries of the Trust. Under the governing documents of the Trust, Warren E. Spieker, Jr. and Carol Spieker share the right to receive and the power to direct the receipt of dividends from, or the proceeds from the sale of, such securities.</ownershipMoreThan5PercentOnBehalfOfAnotherPerson>
      </item6>
      <item7>
        <notApplicableFlag>Y</notApplicableFlag>
      </item7>
      <item8>
        <notApplicableFlag>Y</notApplicableFlag>
      </item8>
      <item9>
        <notApplicableFlag>Y</notApplicableFlag>
      </item9>
      <item10>
        <notApplicableFlag>N</notApplicableFlag>
        <certifications>By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11.</certifications>
      </item10>
    </items>
    <signatureInformation>
      <reportingPersonName>Warren E. Spieker, Jr., individually and as Trustee of the Spieker Living Trust UAD 3/12/02</reportingPersonName>
      <signatureDetails>
        <signature>/s/ Warren E. Spieker, Jr.</signature>
        <title>Warren E. Spieker, Jr., individually and as Trustee of the Spieker Living trust UAD 3/12/02</title>
        <date>08/14/2025</date>
      </signatureDetails>
    </signatureInformation>
  </formData>
</edgarSubmission>
