<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2022-02-02</periodOfReport>

    <noSecuritiesOwned>1</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001822691</issuerCik>
        <issuerName>GreenLight Biosciences Holdings, PBC</issuerName>
        <issuerTradingSymbol>GRNA</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001908721</rptOwnerCik>
            <rptOwnerName>Walker Matthew Alan</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O GREENLIGHT BIOSCIENCES HOLDINGS, PBC</rptOwnerStreet1>
            <rptOwnerStreet2>200 BOSTON AVENUE, SUITE 3100</rptOwnerStreet2>
            <rptOwnerCity>MEDFORD</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02155</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001785504</rptOwnerCik>
            <rptOwnerName>BUILDERS VISION LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>110 N.W. 2ND STREET</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 300</rptOwnerStreet2>
            <rptOwnerCity>BENTONVILLE</rptOwnerCity>
            <rptOwnerState>AR</rptOwnerState>
            <rptOwnerZipCode>72712</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <remarks>Exhibit 24 Power of Attorney filed herewith.
S2G Advisors, LLC has changed its name to Builders Vision, LLC.
Mr. Walker's middle name is spelled Allen, not Alan.</remarks>

    <ownerSignature>
        <signatureName>/s/ David Kennedy, Attorney-in-fact for Matthew Allen Walker</signatureName>
        <signatureDate>2022-02-04</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Lisa J. Forbes, as authorized officer of Builders Vision, LLC</signatureName>
        <signatureDate>2022-02-04</signatureDate>
    </ownerSignature>
</ownershipDocument>
