144 0001901321 XXXXXXXX LIVE 0001761612 BICYCLE THERAPEUTICS PLC 001-38916 BLOCKS A & B, PORTWAY BUILDING GRANTA PARK, GREAT ABINGTON CAMBRIDGE X0 CB21 6GS 011441223261503 Milnes Alistair Officer Common Stock Stifel Nicolaus & Company Inc

501 N Broadway St. Louis MO 63102

2264 19023.00 49868567 10/02/2025 Nasdaq Common Stock 10/02/2025 Restricted Stock Units Issuer N 2437 10/02/2025 Equity Compensation Common Stock 10/03/2025 Restricted Stock Units Issuer N 2031 10/03/2025 Equity Compensation N Alistair Milnes

Blocks A & B Portway Building Cambridge X0 CB21 6GS

Common Stock 07/02/2025 1149 8148.00 Alistair Milnes

Blocks A & B Portway Building Cambridge X0 CB21 6GS

Common Stock 07/03/2025 966 7184.00 10/02/2025 /s/ James Weimer, as a duly authorized representative of STIFEL, as attorney-in-fact for Alistair Milnes