X0508 4 2024-01-02 0 0001761612 BICYCLE THERAPEUTICS plc BCYC 0001901321 Milnes Alistair C/O BICYCLE THERAPEUTICS PLC BLOCKS A & B, PORTWAY BUILDING CAMBRIDGE X0 CB21 6GS UNITED KINGDOM 0 1 0 0 Chief Operating Officer 0 Ordinary Shares 2024-01-02 4 A 0 39000 0.00 A 75839 D Ordinary Shares 2024-01-03 4 S 0 2643 17.50 D 73196 D Employee Stock Option (right to buy) 18.08 2024-01-02 4 A 0 77000 0.00 A 2034-01-02 Ordinary Shares 77000 77000 D Represents a restricted stock unit ("RSU") award. One-fourth (1/4) of the total number of RSUs shall vest on January 2, 2025 and the remaining RSUs shall vest in 12 equal quarterly installments thereafter. Each RSU represents a contingent right to receive one ordinary share. Represents the number of shares required to be sold to cover the statutory tax withholding obligations in connection with the vesting and settlement of the RSUs. This sale is mandated by the Reporting Person's award agreement that requires the satisfaction of minimum statutory tax withholding obligations to be funded by a "sell to cover" transaction and does not represent a discretionary sale by the Reporting Person. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $17.07 to $18.02 inclusive. The Reporting Person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. This option shall vest one-fourth (1/4) of the total number of shares underlying the option on January 2, 2025 and the remaining shares in 36 equal monthly installments thereafter. /s/ Jason Minio, Attorney-in-Fact 2024-01-04