X0206 3/A 2022-01-03 2022-01-05 0 0001761612 BICYCLE THERAPEUTICS plc BCYC 0001901321 Milnes Alistair C/O BICYCLE THERAPEUTICS PLC B900, BABRAHAM RESEARCH CAMPUS CAMBRIDGE X0 CB22 3AT UNITED KINGDOM 0 1 0 0 Chief Operating Officer Ordinary Shares 2325 D These shares were inadvertently omitted from the Reporting Person's initial Form 3 filed on January 5, 2022. /s/ Lee Kalowski, Attorney-in-Fact 2022-03-29