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  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001847337</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001894562</issuerCik>
      <issuerName>Prime Medicine, Inc.</issuerName>
      <secFileNumber>001-41536</secFileNumber>
      <issuerAddress>
        <com:street1>21 ERIE ST.</com:street1>
        <com:city>CAMBRIDGE</com:city>
        <com:stateOrCountry>FL</com:stateOrCountry>
        <com:zipCode>02139</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>617-465-0013</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Alphabet Holdings LLC</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>10% Stockholder</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Raymond James &amp; Associates, Inc.</name>
        <address>
          <com:street1>880</com:street1>
          <com:city>St. Petersburg</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>33716</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>522561</noOfUnitsSold>
      <aggregateMarketValue>7791384.51</aggregateMarketValue>
      <noOfUnitsOutstanding>97227809</noOfUnitsOutstanding>
      <approxSaleDate>06/20/2023</approxSaleDate>
      <securitiesExchangeName>Nasdaq</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>04/01/2021</acquiredDate>
      <natureOfAcquisitionTransaction>Stock Purchase</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>6526586</amountOfSecuritiesAcquired>
      <paymentDate>04/01/2021</paymentDate>
      <natureOfPayment>Cash</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/18/2023</saleDate>
      <amountOfSecuritiesSold>14022</amountOfSecuritiesSold>
      <grossProceeds>183170.79</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/19/2023</saleDate>
      <amountOfSecuritiesSold>63212</amountOfSecuritiesSold>
      <grossProceeds>877066.50</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/22/2023</saleDate>
      <amountOfSecuritiesSold>87922</amountOfSecuritiesSold>
      <grossProceeds>1242091.68</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/23/2023</saleDate>
      <amountOfSecuritiesSold>43939</amountOfSecuritiesSold>
      <grossProceeds>647647.68</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/24/2023</saleDate>
      <amountOfSecuritiesSold>7900</amountOfSecuritiesSold>
      <grossProceeds>107920.32</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/25/2023</saleDate>
      <amountOfSecuritiesSold>23161</amountOfSecuritiesSold>
      <grossProceeds>309972.93</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/26/2023</saleDate>
      <amountOfSecuritiesSold>12602</amountOfSecuritiesSold>
      <grossProceeds>172603.29</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/30/2023</saleDate>
      <amountOfSecuritiesSold>16805</amountOfSecuritiesSold>
      <grossProceeds>230240.26</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>05/31/2023</saleDate>
      <amountOfSecuritiesSold>16633</amountOfSecuritiesSold>
      <grossProceeds>227003.86</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/01/2023</saleDate>
      <amountOfSecuritiesSold>45223</amountOfSecuritiesSold>
      <grossProceeds>649189.73</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/05/2023</saleDate>
      <amountOfSecuritiesSold>22434</amountOfSecuritiesSold>
      <grossProceeds>374611.91</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/06/2023</saleDate>
      <amountOfSecuritiesSold>29975</amountOfSecuritiesSold>
      <grossProceeds>510165.51</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/07/2023</saleDate>
      <amountOfSecuritiesSold>15000</amountOfSecuritiesSold>
      <grossProceeds>253870.50</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/08/2023</saleDate>
      <amountOfSecuritiesSold>5000</amountOfSecuritiesSold>
      <grossProceeds>78686.50</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/09/2023</saleDate>
      <amountOfSecuritiesSold>15672</amountOfSecuritiesSold>
      <grossProceeds>226983.84</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/12/2023</saleDate>
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      <grossProceeds>240150.79</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/13/2023</saleDate>
      <amountOfSecuritiesSold>28100</amountOfSecuritiesSold>
      <grossProceeds>425102.42</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/14/2023</saleDate>
      <amountOfSecuritiesSold>28960</amountOfSecuritiesSold>
      <grossProceeds>430684.43</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/15/2023</saleDate>
      <amountOfSecuritiesSold>66659</amountOfSecuritiesSold>
      <grossProceeds>994325.64</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>34043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/16/2023</saleDate>
      <amountOfSecuritiesSold>6532</amountOfSecuritiesSold>
      <grossProceeds>98109.33</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Alphabet Holdings LLC</name>
        <address>
          <com:street1>1600 Amphitheatre Pkwy</com:street1>
          <com:city>Mountain View</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94043</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>06/20/2023</saleDate>
      <amountOfSecuritiesSold>6532</amountOfSecuritiesSold>
      <grossProceeds>199157.47</grossProceeds>
    </securitiesSoldInPast3Months>
    <noticeSignature>
      <noticeDate>06/20/2023</noticeDate>
      <signature>Tracey Cornelison, as duly authorized representative of Raymond James &amp; Associates, Inc., as attorney-in-fact for Alphabet Holdings LLC</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
