SCHEDULE 13G/A 0001193125-24-257248 0001839947 XXXXXXXX LIVE 3 Common Stock, $0.00001 par value 12/31/2024 0001880438 AN2 Therapeutics, Inc. 037326105 1800 El Camino Real, Suite D Menlo Park CA 94027 Rule 13d-1(c) TCG Crossover GP I, LLC b DE 0 0 0 0 0 N 0 OO TCG Crossover Fund I, L.P. b DE 0 0 0 0 0 N 0 PN Chen Yu b X1 0 0 0 0 0 N 0 IN AN2 Therapeutics, Inc. 1800 El Camino Real, Suite D, Menlo Park, CA 94027 Explanatory Note This Amendment No. 3 (Amendment No. 3) amends and supplements the Schedule 13G initially filed with the Commission on July 25, 2023, as amended by Amendment No. 1 filed with the Commission on February 9, 2024 and Amendment No. 2 filed with the Commission on November 13, 2024 (collectively, the Original Schedule 13G) and is being filed by TCG Crossover Fund I, L.P. (TCG Crossover I) and TCG Crossover GP I, LLC (TCG Crossover GP I and together with TCG Crossover I, the Reporting Entities) and Chen Yu (the Reporting Individual). The Reporting Entities and the Reporting Individual are collectively referred to as the Reporting Persons. The agreement among the Reporting Persons to file jointly in accordance with the provisions of Rule 13d-1(k)(1) under the Act is filed as Exhibit 1 to the Original Schedule 13G. Only those items that are hereby reported are amended; all other items reported in the Original Schedule 13G remain unchanged. Information given in response to each item shall be deemed incorporated by reference in all other items, as applicable. Capitalized terms not defined in this Amendment No. 3 have the meanings ascribed to them in the Original Schedule 13G. The address of the principal business office of each Reporting Person is 705 High St., Palo Alto, CA 94301. TCG Crossover GP I is a limited liability company organized under the laws of the State of Delaware. TCG Crossover I is a limited partnership organized under the laws of the State of Delaware. The Reporting Individual is a citizen of the United States of America. Y See Row 9 of the cover page for each Reporting Person. See Row 11 of the cover page for each Reporting Person. See Row 5 of the cover page for each Reporting Person. See Row 6 of the cover page for each Reporting Person. See Row 7 of the cover page for each Reporting Person. See Row 8 of the cover page for each Reporting Person. N Y N Under certain circumstances set forth in the limited partnership agreements of TCG Crossover I and the limited liability company agreement of TCG Crossover GP I, the general and limited partners or members, as the case may be, of each of such entities may be deemed to have the right to receive dividends from, or the proceeds from, the sale of securities of the Issuer owned by each such entity of which they are a partner or member, as the case may be. Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. TCG Crossover GP I, LLC /s/ Craig Skaling 02/14/2025 TCG Crossover Fund I, L.P. /s/ Craig Skaling 02/14/2025 Chen Yu /s/ Craig Skaling 02/14/2025