<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2022-07-20</periodOfReport>

    <issuer>
        <issuerCik>0001880343</issuerCik>
        <issuerName>Fresh Vine Wine, Inc.</issuerName>
        <issuerTradingSymbol>VINE</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001898432</rptOwnerCik>
            <rptOwnerName>Nechio &amp; Novak, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>505 HIGHWAY 169 NORTH</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 255</rptOwnerStreet2>
            <rptOwnerCity>PLYMOUTH</rptOwnerCity>
            <rptOwnerState>MN</rptOwnerState>
            <rptOwnerZipCode>55441</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>false</isDirector>
            <isOfficer>false</isOfficer>
            <isTenPercentOwner>true</isTenPercentOwner>
            <isOther>false</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock, $.001 par value</value>
            </securityTitle>
            <transactionDate>
                <value>2022-07-20</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>J</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>5317653</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>0</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F2"/>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value></value>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable></derivativeTable>

    <footnotes>
        <footnote id="F1">The reporting person distributed the shares to its members on a pro rata basis.</footnote>
        <footnote id="F2">Nechio &amp; Novak is a Texas limited liability company of which Rick Nechio and Damian Novak are co-founders. Messrs. Nechio and Novak share voting and dispositive power over the shares of common stock of Fresh Vine Wine, Inc. held by Nechio &amp; Novak, LLC. Each of Messrs. Nechio and Novak disclaims beneficial ownership over the shares of common stock of Fresh Vine Wine, Inc. held by Nechio &amp; Novak, LLC except to the extent of his pecuniary interest in such shares.</footnote>
    </footnotes>

    <remarks>Exhibit 24.1 Power of Attorney filed with Form 3 on December 13, 2021 and incorporated herein by reference.</remarks>

    <ownerSignature>
        <signatureName>/s/ Elliot Savoie, Attorney-in-Fact</signatureName>
        <signatureDate>2022-07-29</signatureDate>
    </ownerSignature>
</ownershipDocument>
