144 0001388325 XXXXXXXX LIVE 0001865494 IO Biotech, Inc. 001-41008 OLE MAALOES VEH 3 COPENHAGEN G7 2200 4570702980 Novo Holdings A/S 10% Stockholder 658,809 Morgan Stanley & Co. LLC

1585 Broadway New York NY 10036

658809 988213.50 65880914 05/10/2024 NASDAQGS Common Stock 11/09/2021 Initial Public Offering IO Biotech, Inc. N 1347906 11/09/2021 Conversion of Series B Preferred Stock Y 05/10/2024 /s/ Barbara Fiorini Due, General Counsel, Finance & Operations