<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2022-01-10</periodOfReport>

    <issuer>
        <issuerCik>0001816431</issuerCik>
        <issuerName>Quantum-Si Inc</issuerName>
        <issuerTradingSymbol>QSI</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001864883</rptOwnerCik>
            <rptOwnerName>Stark John VIII</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O QUANTUM-SI INCORPORATED</rptOwnerStreet1>
            <rptOwnerStreet2>530 OLD WHITFIELD STREET</rptOwnerStreet2>
            <rptOwnerCity>GUILFORD</rptOwnerCity>
            <rptOwnerState>CT</rptOwnerState>
            <rptOwnerZipCode>06437</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>true</isDirector>
            <isOfficer>true</isOfficer>
            <officerTitle>Chief Executive Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2022-01-10</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>172167</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>5.8561</value>
                    <footnoteId id="F2"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1985070</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F3"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2022-01-11</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>7702</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>6.2841</value>
                    <footnoteId id="F4"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1977368</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F3"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">The sales reported in this Form 4 were effected to cover tax withholding obligations in connection with the vesting of restricted stock units (&quot;RSUs&quot;) that were granted on February 17, 2021 pursuant to a &quot;sell to cover&quot; provision included in the RSU Agreement.</footnote>
        <footnote id="F2">Represents the weighted average sales price per share. The shares sold at prices ranging from $5.71 to $6.41 per share. Full information regarding the number of shares sold at each price shall be provided upon request to the staff of the U.S. Securities and Exchange Commission, the Issuer, or a security holder of the Issuer.</footnote>
        <footnote id="F3">Includes 1,731,372 RSUs. Each RSU represents the right to receive one share of common stock upon vesting. 425,865 RSUs vested on January 7, 2022, with 1,277,595 RSUs vesting in 12 equal quarterly installments thereafter beginning with the quarter ending March 31, 2022, subject to Mr. Stark's continued service through the applicable vesting date. 453,777 RSUs vest (i) on the closing of a financing in excess of $50 million within three years of Mr. Stark's start date at a share price greater than $16.08 (as adjusted), or (ii) if the Issuer is a publicly listed company and within three years of Mr. Stark's start date the closing price of the Issuer's shares is $16.08 (as adjusted) or more for any 20 trading days within any 30 consecutive trading day period, subject to Mr. Stark's continued service through the applicable vesting date.</footnote>
        <footnote id="F4">Represents the weighted average sales price per share. The shares sold at prices ranging from $6.25 to $6.34 per share. Full information regarding the number of shares sold at each price shall be provided upon request to the staff of the U.S. Securities and Exchange Commission, the Issuer, or a security holder of the Issuer.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Christian LaPointe, Ph.D., Attorney-in-Fact</signatureName>
        <signatureDate>2022-01-12</signatureDate>
    </ownerSignature>
</ownershipDocument>
