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        <name>NASSAU CNTY NY TOBACCO SETTLEM</name>
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        <name>CHICAGO IL BRD OF EDU</name>
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          <maturityDt>2041-12-01</maturityDt>
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          <annualizedRt>4.00000000</annualizedRt>
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      <invstOrSec>
        <name>MASSACHUSETTS ST HSG FIN AGY H</name>
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        <title>Massachusetts Housing Finance Agency</title>
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          <isin value="US57587GKG90"/>
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        <valUSD>3119240.00000000</valUSD>
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          <maturityDt>2051-12-01</maturityDt>
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          <annualizedRt>2.87500000</annualizedRt>
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      <invstOrSec>
        <name>BUCKEYE OH TOBACCO SETTLEMENT</name>
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        <title>Buckeye Tobacco Settlement Financing Authority</title>
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          <isin value="US118217CZ97"/>
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        <name>PUERTO RICO ELEC PWR AUTH PWR</name>
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        <name>SUFFOLK NY TOBACCO ASSET SECUR</name>
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        <name>FLORIDA ST TURNPIKE AUTH TURNP</name>
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        <name>GOLDEN ST TOBACCO SECURITIZATI</name>
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        <name>MET PIER &amp; EXPOSITION AUTH IL</name>
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        <name>CHICAGO IL BRD OF EDU</name>
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        <name>HARRIS CNTY TX CULTURAL EDU FA</name>
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      <invstOrSec>
        <name>NEW YORK ST TRANSPRTN DEV CORP</name>
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          <annualizedRt>2.12500000</annualizedRt>
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        <name>PHILADELPHIA PA AUTH FOR INDL</name>
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        <name>HOOVER AL INDL DEV BRD ENVNMNT</name>
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        <name>NEW YORK CNTYS NY TOBACCO TRUS</name>
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        <lei>N/A</lei>
        <title>Arkansas Development Finance Authority</title>
        <cusip>04108WCC4</cusip>
        <identifiers>
          <isin value="US04108WCC47"/>
        </identifiers>
        <balance>8360000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>8099753.20000000</valUSD>
        <pctVal>3.378349579240</pctVal>
        <payoffProfile>Long</payoffProfile>
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        <issuerCat>MUN</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2049-09-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>4.50000000</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Housing Opportunities Comm of</name>
        <lei>549300HDL0LLRHMDL092</lei>
        <title>Housing Opportunities Commission of Montgomery County</title>
        <cusip>613347PM9</cusip>
        <identifiers>
          <isin value="US613347PM90"/>
        </identifiers>
        <balance>1500000.00000000</balance>
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        <curCd>USD</curCd>
        <valUSD>1058355.00000000</valUSD>
        <pctVal>0.441432359807</pctVal>
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        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2052-07-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>2.10000000</annualizedRt>
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          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ERIE CNTY NY TOBACCO ASSET SEC</name>
        <lei>N/A</lei>
        <title>Erie Tobacco Asset Securitization Corp</title>
        <cusip>295095BB8</cusip>
        <identifiers>
          <isin value="US295095BB81"/>
        </identifiers>
        <balance>4385000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>4366363.75000000</valUSD>
        <pctVal>1.821179333911</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>DBT</assetCat>
        <issuerCat>MUN</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2045-06-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>5.00000000</annualizedRt>
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          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ARKANSAS ST DEV FIN AUTH INDL</name>
        <lei>N/A</lei>
        <title>Arkansas Development Finance Authority</title>
        <cusip>04108WCD2</cusip>
        <identifiers>
          <isin value="US04108WCD20"/>
        </identifiers>
        <balance>2500000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>2506575.00000000</valUSD>
        <pctVal>1.045474644410</pctVal>
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        <assetCat>DBT</assetCat>
        <issuerCat>MUN</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2049-09-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>4.75000000</annualizedRt>
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          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>NEW JERSEY ST HLTH CARE FACS F</name>
        <lei>N/A</lei>
        <title>New Jersey Health Care Facilities Financing Authority</title>
        <cusip>645790PE0</cusip>
        <identifiers>
          <isin value="US645790PE03"/>
        </identifiers>
        <balance>1715000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>1389493.00000000</valUSD>
        <pctVal>0.579547669663</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>DBT</assetCat>
        <issuerCat>MUN</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2051-07-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>3.00000000</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>CONROE TX LOCAL GOVT CORP HOTE</name>
        <lei>N/A</lei>
        <title>Conroe Local Government Corp</title>
        <cusip>208419AD3</cusip>
        <identifiers>
          <isin value="US208419AD33"/>
        </identifiers>
        <balance>1000000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>887050.00000000</valUSD>
        <pctVal>0.369982259986</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>DBT</assetCat>
        <issuerCat>MUN</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2050-10-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>5.00000000</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>HOUSTON TX ARPT SYS REVENUE</name>
        <lei>N/A</lei>
        <title>City of Houston TX Airport System Revenue</title>
        <cusip>442349GA8</cusip>
        <identifiers>
          <isin value="US442349GA89"/>
        </identifiers>
        <balance>18130000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>16144221.10000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>DBT</assetCat>
        <issuerCat>MUN</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2041-07-15</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>4.00000000</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>AEROTROPOLIS REGL TRANPRTN AUT</name>
        <lei>N/A</lei>
        <title>Aerotropolis Regional Transportation Authority</title>
        <cusip>008051AC8</cusip>
        <identifiers>
          <isin value="US008051AC80"/>
        </identifiers>
        <balance>2000000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>1733160.00000000</valUSD>
        <pctVal>0.722888736505</pctVal>
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        <assetCat>DBT</assetCat>
        <issuerCat>MUN</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2052-12-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>4.37500000</annualizedRt>
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          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>SOUTH BROWARD FL HOSP DIST</name>
        <lei>N/A</lei>
        <title>South Broward Hospital District</title>
        <cusip>836753NK5</cusip>
        <identifiers>
          <isin value="US836753NK56"/>
        </identifiers>
        <balance>2455000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>1804130.40000000</valUSD>
        <pctVal>0.752489986699</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>DBT</assetCat>
        <issuerCat>MUN</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2047-05-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>2.50000000</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <signature>
      <ncom:dateSigned>2022-09-23</ncom:dateSigned>
      <ncom:nameOfApplicant>Pioneer Municipal High Income Opportunities Fund, Inc.</ncom:nameOfApplicant>
      <ncom:signature>Anthony J. Koenig, Jr</ncom:signature>
      <ncom:signerName>Anthony J. Koenig, Jr</ncom:signerName>
      <ncom:title>Managing Director, Chief Operations Officer &amp; Treasurer of the Funds</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
