144 0001668085 XXXXXXXX LIVE 0001858848 Tenaya Therapeutics, Inc. 001-40656 171 OYSTER POINT BLVD., SUITE 500 SOUTH SAN FRANCISCO CA 94080 415-865-2066 COLUMN GROUP III-A, LP 10% Stockholder Common BTIG, LLC

350 Bush Street, 9th floor San Francisco CA 94104

1060727 596552.86 213744535 02/25/2026 NASDAQ GS Common 08/30/2019 Series B financing Tenaya Therapeutics N 177927 08/30/2019 Cash Common 12/20/2018 Series A financing Tenaya Therapeutics N 313640 12/20/2018 Cash Common 03/30/2020 Series B financing Tenaya Therapeutics N 177927 03/30/2020 Cash Common 08/25/2020 Series B financing Tenaya Therapeutics N 177927 08/25/2020 Cash Common 12/17/2020 Series B financing Tenaya Therapeutics N 213306 12/17/2020 Cash N COLUMN GROUP III-A, LP

1 Letterman Drive Suite DM-900 San Francisco CA 94129

Common 02/10/2026 182295 148770.95 COLUMN GROUP III-A, LP

1 Letterman Drive, Suite DM-900 San Francisco CA 94129

Common 02/11/2026 2151458 1464712.61 COLUMN GROUP III-A, LP

1 Letterman Drive, Suite DM-900 San Francisco CA 94129

Common 02/20/2026 277769 170800.16 COLUMN GROUP III-A, LP

1 Letterman Drive, Suite DM-900 San Francisco CA 94129

Common 02/23/2026 162291 96920.19 COLUMN GROUP III-A, LP

1 Letterman Drive, Suite DM-900 San Francisco CA 94129

Common 02/24/2026 349212 214870.14 02/25/2026 James Evangelista