<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2025-03-14</periodOfReport>

    <notSubjectToSection16>true</notSubjectToSection16>

    <issuer>
        <issuerCik>0001856028</issuerCik>
        <issuerName>Stronghold Digital Mining, Inc.</issuerName>
        <issuerTradingSymbol>SDIG</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001886625</rptOwnerCik>
            <rptOwnerName>Pacchia Thomas</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>595 MADISON AVENUE, 28TH FLOOR</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>true</isDirector>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A common stock, par value $0.0001</value>
            </securityTitle>
            <transactionDate>
                <value>2025-03-14</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>D</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>67226</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                </transactionShares>
                <transactionPricePerShare>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>0</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Represents shares of Class A common stock, par value $0.0001 per share (&quot;Class A common stock&quot;), of Stronghold Digital Mining, Inc. (&quot;Stronghold&quot;) disposed of pursuant to the closing of the transactions contemplated by the Agreement and Plan of Merger (the &quot;initial merger agreement&quot;), dated August 21, 2024, by and among Stronghold, Bitfarms Ltd. (&quot;Bitfarms&quot;), Backbone Mining Solutions LLC, an indirect, wholly-owned subsidiary of Bitfarms (&quot;BMS&quot;) and HPC &amp; AI Megacorp, Inc., a direct, wholly-owned subsidiary of BMS (&quot;Merger Sub&quot;), which was amended by amendment no. 1 thereto dated as of September 12, 2024 (&quot;amendment no. 1,&quot; and together with the initial merger agreement, the &quot;Merger Agreement&quot;).</footnote>
        <footnote id="F2">(continued) At the Effective Time (as defined in the Merger Agreement), Stronghold merged with and into Merger Sub with Stronghold continuing as the surviving corporation, and each share of Class A common stock issued and outstanding immediately prior to the Effective Time was converted into the right to receive 2.52 Bitfarms common shares, no par value (&quot;Bitfarms common shares&quot;), with any fractional shares paid out in cash.</footnote>
        <footnote id="F3">On March 13, 2025, the last trading day prior to the Effective Time, the closing price of one Bitfarms common share was $1.09.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Matthew Usdin, Attorney-in-Fact for Thomas Pacchia</signatureName>
        <signatureDate>2025-03-18</signatureDate>
    </ownerSignature>
</ownershipDocument>
