Exhibit 99.1

Teknova Reports Second Quarter 2025 Financial Results

Second quarter 2025 total revenue was $10.3 million, up 7% from prior year

Achieves four consecutive quarters of year-over-year revenue growth

Company reaffirms 2025 revenue guidance of $39-42 million

HOLLISTER, Calif., August 7, 2025 – Alpha Teknova, Inc. (“Teknova” or the “Company”) (Nasdaq: TKNO), a leading producer of critical reagents for the discovery, development, and commercialization of novel therapies, vaccines, and molecular diagnostics, today announced financial results for the second quarter ended June 30, 2025.

“Despite continued uncertainty in the macroenvironment, we delivered another strong quarter of results, with improvements in both the top and bottom lines,” said Stephen Gunstream, President and Chief Executive Officer at Teknova. “We continue to believe that the investments we’ve made in our operational and commercial infrastructure, combined with our diverse product portfolio and customer base, position Teknova for sustainable, above-market growth over the long-term.”

Matt Lowell, Teknova’s Chief Financial Officer, added, “Compared to 2024, we delivered 7% revenue growth in the second quarter 2025 along with significant improvements in adjusted EBITDA and free cash outflow. We believe the Company is well positioned to maintain its momentum. We therefore reiterate our 2025 revenue guidance of $39-42 million and continue to anticipate full-year free cash outflow of less than $12 million,” he explained.

Corporate and Financial Updates

•Second quarter 2025 total revenue of $10.3 million, up 7% from the second quarter 2024

•Revenue was up year-over-year for each of the past four consecutive quarters

•Total cash and short-term investments were $24.0 million and total borrowings were $13.2 million at the end of the second quarter 2025

Revenue for the Second Quarter 2025

	For the Three Months Ended June 30,				For the Six Months Ended June 30,
(Dollars in thousands)	2025		2024		2025		2024
Lab Essentials	$	7,792	$	7,638	$	15,909	$	14,904
Clinical Solutions		2,060		1,565		3,222		3,283
Other		435		411		951		717
Total revenue	$	10,287	$	9,614	$	20,082	$	18,904

Second Quarter 2025 Financial Results

Total revenue for the second quarter 2025 was $10.3 million, up 7% compared to $9.6 million in the second quarter 2024. Lab Essentials revenue was $7.8 million in the second quarter 2025, up 2% compared to $7.6

million in the second quarter 2024. Clinical Solutions revenue was $2.1 million in the second quarter 2025, up 32% compared to $1.6 million in the second quarter 2024.

Gross profit for the second quarter 2025 was $4.0 million, compared to $2.8 million in the second quarter 2024. Gross margin for the second quarter 2025 was 38.7%, compared to 29.2% in the second quarter 2024. The increase in gross margin was driven by manufacturing efficiency gains and higher revenue.

Operating expenses for the second quarter 2025 were $7.4 million, compared to $7.9 million in the second quarter 2024. Excluding a non-recurring charge of $0.1 million recorded in the second quarter of 2024 related to a loss contingency, operating expenses were down $0.5 million. The decrease was driven by reduced spending, primarily on insurance and facility costs.

Net loss for the second quarter 2025 was $3.6 million, or negative $0.07 per diluted share, compared to $5.4 million, or negative $0.13 per diluted share, for the second quarter 2024.

Adjusted EBITDA for the second quarter 2025 was negative $0.8 million, compared to negative $2.6 million for the second quarter 2024. Free Cash Flow was negative $2.3 million for the second quarter 2025, compared to negative $3.0 million for the second quarter 2024. A full reconciliation of these non-GAAP measures to the most comparable GAAP measures is included at the end of this release.

Reiterates 2025 Outlook

Teknova reiterates its fiscal 2025 outlook for revenue and free cash outflow. The Company continues to anticipate total revenue of $39 million to $42 million for the fiscal year ending December 31, 2025. The Company also anticipates free cash outflow of less than $12 million for 2025.

Conference Call and Webcast

Teknova will host a webcast and conference call on Thursday, August 7, 2025, beginning at 5:00 p.m. Eastern Time. To access the live webcast, listeners can log onto the call from the Investor Relations section of the Teknova website or by using this link. If you would like to participate in the call, please register for the webcast here to receive a unique PIN number and dial-in information. The webcast will be available for replay on the Company’s website approximately two hours after the event.

About Teknova

Teknova makes solutions possible. Since 1996, Teknova has been innovating the manufacture of critical reagents for the life sciences industry to accelerate the discovery and development of novel breakthroughs that will help people live longer, healthier lives. We offer fully customizable solutions for every stage of the workflow, supporting industry leaders in molecular diagnostics, synthetic biology, and emerging therapeutic modalities. Our fast turnaround of high-quality agar plates, microbial culture and cryopreservation media, buffers and reagents, and water helps our customers scale seamlessly from RUO to GMP. Headquartered in Hollister, California, with over 180,000 square feet of state-of-the-art facilities, Teknova’s modular manufacturing platform was designed by our team of scientists, engineers, and quality control experts to efficiently produce the foundational ingredients for the discovery and commercialization of next-generation therapies.

Non-GAAP Financial Measures

This press release contains financial measures that have not been calculated in accordance with U.S. generally accepted accounting principles (GAAP). Teknova uses the following non-GAAP financial measures in assessing the performance of its business and the effectiveness of its business strategies: (a) Adjusted EBITDA and (b) Free Cash Flow.

Teknova defines Adjusted EBITDA as net loss adjusted for interest income (expense), net, provision for (benefit from) income taxes, depreciation expense, amortization of intangible assets, and stock-based compensation expense. Adjusted EBITDA reflects further adjustments to eliminate the impact of certain items, including certain non-cash and other items that Teknova does not consider representative of its ongoing operating performance.

Teknova continues to define Free Cash Flow as cash used in operating activities plus purchases of property, plant, and equipment.

Teknova provides Adjusted EBITDA and Free Cash Flow in this press release because Teknova believes that analysts, investors, and other interested parties frequently use these measures to evaluate companies in Teknova’s industry and that such measures facilitate comparisons on a consistent basis across reporting periods. Teknova also believes such measures are helpful in highlighting trends in Teknova’s operating results because they exclude items that are not indicative of Teknova’s core operating performance. Investors should consider non-GAAP financial measures in addition to, and not as a substitute for, or as superior to, measures of financial performance prepared in accordance with GAAP. The non-GAAP financial measures presented by Teknova may be different from the non-GAAP financial measures used by other companies.

A full reconciliation of these non-GAAP measures to the most comparable GAAP measures is included at the end of this release.

Forward-Looking Statements

Statements in this press release about future expectations, plans, and prospects, as well as any other statements regarding matters that are not historical facts, may constitute “forward-looking statements.” These statements include, but are not limited to, statements relating to Teknova’s anticipated total revenue, including expectations for 2025 revenue and free cash outflow guidance, and other statements about Teknova’s business prospects, including about Teknova’s profitability, strategy of managing operating expenses, and long-term growth strategy. The words, without limitation, “anticipate,” “believe,” “continue,” “could,” “estimate,” “expect,” “intend,” “may,” “plan,” “potential,” “predict,” “project,” “should,” “target,” “will,” “would,” and similar expressions are intended to identify forward-looking statements, although not all forward-looking statements contain these or similar identifying words. These forward-looking statements are based on management’s current expectations and beliefs and are subject to risks and uncertainties, all of which are difficult to predict and many of which are beyond Teknova’s control and could cause actual results to differ materially and adversely from those described in the forward-looking statements. These risks and uncertainties include, but are not limited to, demand for Teknova’s products (including the potential delay to or pausing of customer orders); Teknova’s assessment of fundamental indicators of future demand across its target customer base; Teknova’s cash flows and revenue growth rate; Teknova’s supply chain, sourcing, manufacturing, and warehousing; inventory management; risks related to global economic and marketplace uncertainties, including those related to the conflicts in Ukraine and the Middle East; reliance on a limited number of customers for a high percentage of Teknova’s revenue; potential acquisitions and integration of other companies; and other factors discussed in the “Risk Factors” section of Teknova’s most recent periodic

reports filed with the Securities and Exchange Commission (“SEC”), including in Teknova’s Annual Report on Form 10-K for the year ended December 31, 2024, and subsequent Quarterly Reports on Form 10-Q filed with the SEC, all of which you may obtain for free on the SEC’s website at www.sec.gov. Although Teknova believes that the expectations reflected in its forward-looking statements are reasonable, Teknova does not know whether its expectations will prove correct. You are cautioned not to place undue reliance on these forward-looking statements, which speak only as of the date hereof, even if subsequently made available by Teknova on its website or otherwise. Teknova does not undertake any obligation to update, amend, or clarify these forward-looking statements, whether as a result of new information, future events, or otherwise, except as may be required under applicable securities laws.

Investor Contact

Matt Lowell

Chief Financial Officer

matt.lowell@teknova.com

+1 831-637-1100

Media Contact

Jennifer Henry

Senior Vice President, Marketing

jenn.henry@teknova.com

+1 831-313-1259

ALPHA TEKNOVA, INC.

Condensed Statements of Operations

(Unaudited)

(In thousands, except share and per share data)

	For the Three Months Ended June 30,						For the Six Months Ended June 30,
	2025			2024			2025			2024
Revenue	$	10,287		$	9,614		$	20,082		$	18,904
Cost of sales		6,303			6,810			13,091			13,891
Gross profit		3,984			2,804			6,991			5,013
Operating expenses:
Research and development		581			678			1,133			1,538
Sales and marketing		1,573			1,456			3,213			3,123
General and administrative		4,929			5,483			10,421			12,864
Amortization of intangible assets		287			287			574			574
Total operating expenses		7,370			7,904			15,341			18,099
Loss from operations		(3,386	)		(5,100	)		(8,350	)		(13,086	)
Other income (expenses), net
Interest expense, net		(165	)		(272	)		(309	)		(417	)
Other adjustment to loan exit fee		—			—			485			—
Total other income (expenses), net		(165	)		(272	)		176			(417	)
Loss before income taxes		(3,551	)		(5,372	)		(8,174	)		(13,503	)
Provision for (benefit from) income taxes		19			(8	)		41			(42	)
Net loss	$	(3,570	)	$	(5,364	)	$	(8,215	)	$	(13,461	)
Net loss per share—basic and diluted	$	(0.07	)	$	(0.13	)	$	(0.15	)	$	(0.33	)
Weighted average shares used in computing net loss per share—basic and diluted		53,448,736			40,853,882			53,435,210			40,829,383

ALPHA TEKNOVA, INC.

Condensed Balance Sheets

(Unaudited)

(In thousands)

	As of June 30,			As of December 31,
	2025			2024
ASSETS
Current assets:
Cash and cash equivalents	$	3,276		$	3,708
Short-term investments, held -to-maturity		20,724			26,688
Accounts receivable, net		5,140			4,312
Inventories, net		7,610			6,801
Prepaid expenses and other current assets		1,307			1,267
Total current assets		38,057			42,776
Property, plant, and equipment, net		43,605			45,753
Operating right-of-use lease assets		14,960			15,767
Intangible assets, net		12,517			13,091
Other non-current assets		1,348			1,382
Total assets	$	110,487		$	118,769
LIABILITIES AND STOCKHOLDERS’ EQUITY
Current liabilities:
Accounts payable	$	1,204		$	825
Accrued liabilities		3,388			4,541
Current portion of operating lease liabilities		1,886			1,800
Current portion of long-term debt		—			4,045
Total current liabilities		6,478			11,211
Deferred tax liabilities		868			827
Other accrued liabilities		—			10
Long-term debt, net		13,032			9,443
Long-term operating lease liabilities		14,052			14,884
Total liabilities		34,430			36,375
Stockholders’ equity:
Preferred stock		—			—
Common stock		1			1
Additional paid-in capital		202,802			200,924
Accumulated deficit		(126,746	)		(118,531	)
Total stockholders’ equity		76,057			82,394
Total liabilities and stockholders’ equity	$	110,487		$	118,769

ALPHA TEKNOVA, INC.

Condensed Statements of Cash Flows

(Unaudited)

(In thousands)

	For the Three Months Ended June 30,						For the Six Months Ended June 30,
	2025			2024			2025			2024
Operating activities:
Net loss	$	(3,570	)	$	(5,364	)	$	(8,215	)	$	(13,461	)
Adjustments to reconcile net loss to net cash used in operating activities:
Bad debt expense		4			49			49			56
Inventory reserve		488			987			925			896
Depreciation and amortization		1,590			1,626			3,170			3,262
Stock-based compensation		950			833			1,802			2,140
Deferred taxes		20			(9	)		41			(44	)
Accrued interest income on short-term investments		108			—			54			—
Amortization of discount on short-term investments		(171	)		—			(355	)		—
Amortization of debt financing costs		43			104			129			188
Other adjustment to loan exit fee		—			—			(485	)		—
Non-cash lease expense		31			47			61			94
Loss on disposal of property, plant, and equipment		19			—			19			49
Changes in operating assets and liabilities:
Accounts receivable		472			(86	)		(877	)		(705	)
Inventories		(1,525	)		(767	)		(1,734	)		(289	)
Prepaid expenses and other current assets		(36	)		239			(40	)		413
Other non-current assets		(29	)		89			34			206
Accounts payable		(360	)		(522	)		380			(389	)
Accrued liabilities		(135	)		(40	)		(1,152	)		(1,764	)
Other		—			(24	)		(10	)		(48	)
Cash used in operating activities		(2,101	)		(2,838	)		(6,204	)		(9,396	)
Investing activities:
Purchases of short-term investments		(7,765	)		—			(9,735	)		—
Maturities of short-term investments		10,000			—			16,000			—
Proceeds from sale of property, plant, and equipment		—			—			—			125
Purchases of property, plant, and equipment		(207	)		(115	)		(413	)		(227	)
Cash provided by (used in) investing activities		2,028			(115	)		5,852			(102	)
Financing activities:
Proceeds from long-term debt		—			—			1,110			—
Payment of exit fee costs		—			—			(1,110	)		—
Payments related to equity financing		—			—			—			(37	)
Repayment of financed insurance premiums		—			(103	)		(56	)		(409	)
Proceeds from exercise of stock options		16			—			20			—
Proceeds from issuance of common stock under employee stock purchase plan		56			81			56			81
Payment of debt issuance costs		(100	)		(25	)		(100	)		(25	)
Cash used in financing activities		(28	)		(47	)		(80	)		(390	)
Change in cash and cash equivalents		(101	)		(3,000	)		(432	)		(9,888	)
Cash and cash equivalents at beginning of period		3,377			21,596			3,708			28,484
Cash and cash equivalents at end of period	$	3,276		$	18,596		$	3,276		$	18,596

ALPHA TEKNOVA, INC.

Reconciliation of Non-GAAP Measures to the Most Comparable GAAP Measures

(Unaudited)

(In thousands)

	For the Three Months Ended June 30,						For the Six Months Ended June 30,
	2025			2024			2025			2024
Net loss – as reported	$	(3,570	)	$	(5,364	)	$	(8,215	)	$	(13,461	)
Add back:
Interest expense, net		(165	)		(272	)		(309	)		(417	)
Provision for (benefit from) income taxes		19			(8	)		41			(42	)
Depreciation expense		1,303			1,339			2,596			2,688
Amortization of intangible assets		287			287			574			574
EBITDA	$	(1,796	)	$	(3,474	)	$	(4,695	)	$	(9,824	)
Other and non-recurring expenses:
Stock-based compensation expense		950			833			1,802			2,140
Severance pay and other termination benefits		—			—			—			1,287
Other adjustment to loan exit fee		—			—			(485	)		—
Loss contingency		—			73			—			73
Adjusted EBITDA	$	(846	)	$	(2,568	)	$	(3,378	)	$	(6,324	)

	For the Three Months Ended June 30,						For the Six Months Ended June 30,
	2025			2024			2025			2024
Cash used in operating activities	$	(2,101	)	$	(2,838	)	$	(6,204	)	$	(9,396	)
Purchases of property, plant, and equipment		(207	)		(115	)		(413	)		(227	)
Free Cash Flow	$	(2,308	)	$	(2,953	)	$	(6,617	)	$	(9,623	)