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    <submissionType>SCHEDULE 13G/A</submissionType>
		<previousAccessionNumber>0001104659-24-119006</previousAccessionNumber>
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      <liveTestFlag>LIVE</liveTestFlag>





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		<amendmentNo>4</amendmentNo>
	      <securitiesClassTitle>COMMON STOCK, $0.0001 PAR VALUE</securitiesClassTitle>
      <eventDateRequiresFilingThisStatement>03/31/2026</eventDateRequiresFilingThisStatement>
      <issuerInfo>
        <issuerCik>0001845337</issuerCik>        <issuerName>Day One Biopharmaceuticals, Inc.</issuerName>

    <issuerCusips>
					<issuerCusipNumber>23954D109</issuerCusipNumber>
				</issuerCusips>

        <issuerPrincipalExecutiveOfficeAddress>
          <com:street1>1800 Sierra Point Parkway, Suite 200</com:street1>
                    <com:city>BRISBANE</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94005</com:zipCode>
        </issuerPrincipalExecutiveOfficeAddress>
      </issuerInfo>

	        <designateRulesPursuantThisScheduleFiled>
	   				        <designateRulePursuantThisScheduleFiled>Rule 13d-1(c)</designateRulePursuantThisScheduleFiled>
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    <items>
      <item1>
        <issuerName>Day One Biopharmaceuticals, Inc.</issuerName>
        <issuerPrincipalExecutiveOfficeAddress>1800 Sierra Point Parkway, Suite 200, BRISBANE, CA, 94005.</issuerPrincipalExecutiveOfficeAddress>
      </item1>

      <item2>
        <filingPersonName>This Schedule 13G is filed by (i) Atlas Venture Fund XI, L.P., a Delaware limited partnership ("Atlas XI"), (ii) Atlas Venture Associates XI, L.P., a Delaware limited partnership ("AVA XI LP"), (iii) Atlas Venture Associates XI, LLC, a Delaware limited liability company ("AVA XI LLC" and together with Atlas XI and AVA XI LP, the "Fund XI Reporting Persons"), (iv) Atlas Venture Opportunity Fund I, L.P., a Delaware limited partnership ("AVO I"), (v) Atlas Venture Associates Opportunity I, L.P., a Delaware limited partnership ("AVAO LP"), (vi) Atlas Venture Associates Opportunity I, LLC, a Delaware limited liability company ("AVAO LLC" and together with AVO I and AVAO LP, the "Opportunity Fund Reporting Persons"), (vii) Atlas Venture Opportunity Fund II, L.P., a Delaware limited partnership ("AVO II"), (viii) Atlas Venture Associates Opportunity II, L.P., a Delaware limited partnership ("AVAO II LP") and (ix) Atlas Venture Associates Opportunity II, LLC, a Delaware limited liability company ("AVAO II LLC" and together with AVO II and AVAO II LP, the "Opportunity Fund II Reporting Persons" and together with the Fund XI Reporting Persons and Opportunity Fund Reporting Persons, the "Reporting Persons").</filingPersonName>        <principalBusinessOfficeOrResidenceAddress>300 Technology Square, 8th Floor
Cambridge, Massachusetts 02139</principalBusinessOfficeOrResidenceAddress>        <citizenship>Each of Atlas XI, AVA XI LP, AVO I, AVAO LP, AVO II and AVAO II LP is a Delaware limited partnership.
Each of AVA XI LLC, AVAO LLC and AVAO II LLC is a Delaware limited liability company.</citizenship>      </item2>

        <item3>
		        <notApplicableFlag>Y</notApplicableFlag>

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            <notApplicableFlag>N</notApplicableFlag>
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	  	  <notApplicableFlag>Y</notApplicableFlag>
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	  	  <notApplicableFlag>Y</notApplicableFlag>


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                <notApplicableFlag>Y</notApplicableFlag>

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            <item9>
                <notApplicableFlag>Y</notApplicableFlag>
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      <item10>
                <notApplicableFlag>N</notApplicableFlag>
        <certifications>By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11.</certifications>

      </item10>
    </items>

		<exhibitInfo>JOINT FILING AGREEMENT</exhibitInfo>

	    <signatureInformation>
      <reportingPersonName>Atlas Venture Fund XI, L.P. </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>By: Atlas Venture Associates XI, L.P., its GP, By: Atlas Venture Associates XI, LLC, its GP, By: Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

    </signatureInformation>
	     <signatureInformation>
      <reportingPersonName>Atlas Venture Associates XI, L.P. </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>By: Atlas Venture Associates XI, LLC, its GP, By: Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

    </signatureInformation>
	     <signatureInformation>
      <reportingPersonName>Atlas Venture Associates XI, LLC </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

    </signatureInformation>
	     <signatureInformation>
      <reportingPersonName>Atlas Venture Opportunity Fund I, L.P. </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>By: Atlas Venture Associates Opportunity I, L.P., its GP, By: Atlas Venture Associates Opportunity I, LLC, its GP, By: Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

    </signatureInformation>
	     <signatureInformation>
      <reportingPersonName>Atlas Venture Associates Opportunity I, L.P. </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>By: Atlas Venture Associates Opportunity I, LLC, its GP, By: Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

    </signatureInformation>
	     <signatureInformation>
      <reportingPersonName>Atlas Venture Associates Opportunity I, LLC </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

    </signatureInformation>
	     <signatureInformation>
      <reportingPersonName>Atlas Venture Opportunity Fund II, L.P. </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>By: Atlas Venture Associates Opportunity II, L.P., its GP, By: Atlas Venture Associates Opportunity II, LLC, its GP, By: Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

    </signatureInformation>
	     <signatureInformation>
      <reportingPersonName>Atlas Venture Associates Opportunity II, L.P. </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>By: Atlas Venture Associates Opportunity II, LLC, its GP, By: Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

    </signatureInformation>
	     <signatureInformation>
      <reportingPersonName>Atlas Venture Associates Opportunity II, LLC </reportingPersonName>

      <signatureDetails>
        <signature>/s/ Ommer Chohan</signature>
        <title>Ommer Chohan, CFO</title>
        <date>05/14/2026</date>
      </signatureDetails>

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