<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <headerData>
    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001843263</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>


      <seriesClassInfo>
        <seriesId>S000071479</seriesId>
        <classId>C000226698</classId>
        <classId>C000226697</classId>
      </seriesClassInfo>


    </filerInfo>
  </headerData>
  <formData>
    <genInfo>
      <regName>Tactical Investment Series Trust</regName>
      <regFileNumber>811-23631</regFileNumber>
      <regCik>0001843263</regCik>
      <regLei>5493006GAMP0G864BR33</regLei>
      <regStreet1>11726 Seven Gables Road</regStreet1>
      <regCity>Cincinnati</regCity>
      <regStateConditional regCountry="US" regState="US-OH"/>
      <regZipOrPostalCode>45249</regZipOrPostalCode>
      <regPhone>631-470-2688</regPhone>
      <seriesName>TFA Quantitative Fund</seriesName>
      <seriesId>S000071479</seriesId>
      <seriesLei>54930029OKZ3T8LPU649</seriesLei>
      <repPdEnd>2023-12-31</repPdEnd>
      <repPdDate>2023-12-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
      <totAssets>40210333.3</totAssets>
      <totLiabs>89082.31</totLiabs>
      <netAssets>40121250.99</netAssets>
      <assetsAttrMiscSec>0</assetsAttrMiscSec>
      <assetsInvested>0</assetsInvested>
      <amtPayOneYrBanksBorr>0</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0</amtPayAftOneYrOther>
      <delayDeliv>0</delayDeliv>
      <standByCommit>0</standByCommit>
      <liquidPref>0</liquidPref>
      <cshNotRptdInCorD>93369.47</cshNotRptdInCorD>
      <curMetrics>
        <curMetric>
          <curCd>USD</curCd>
          <intrstRtRiskdv01 period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
          <intrstRtRiskdv100 period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
        </curMetric>
      </curMetrics>
      <creditSprdRiskInvstGrade period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
      <creditSprdRiskNonInvstGrade period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000226698" rtn1="N/A" rtn2="N/A" rtn3="N/A"/>
          <monthlyTotReturn classId="C000226697" rtn1="-2.02" rtn2="5.94" rtn3="4.49"/>
        </monthlyTotReturns>
        <othMon1 netRealizedGain="-130152.85" netUnrealizedAppr="-390383.72"/>
        <othMon2 netRealizedGain="-219503.88" netUnrealizedAppr="1822670.32"/>
        <othMon3 netRealizedGain="257134.79" netUnrealizedAppr="1373895.87"/>
      </returnInfo>
      <mon1Flow redemption="1179895.01" reinvestment="0" sales="1388643.73"/>
      <mon2Flow redemption="1169170.6" reinvestment="0" sales="11475276.78"/>
      <mon3Flow redemption="904206.21" reinvestment="12243.31" sales="691586.1"/>


    </fundInfo>
    <invstOrSecs>
      <invstOrSec>
        <name>AGF US Market Neutral Anti-Beta Fund</name>
        <lei>549300YBDDPCJBGJCO29</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>00110G408</cusip>
        <identifiers>
          <isin value="US00110G4082"/>
        </identifiers>
        <balance>20400</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>345780</valUSD>
        <pctVal>0.00862</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>FT Vest Fund of Buffer ETFs</name>
        <lei>549300OCPQ9IXM4SN419</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>33740F755</cusip>
        <identifiers>
          <isin value="US33740F7556"/>
        </identifiers>
        <balance>90200</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2396614</valUSD>
        <pctVal>0.05973</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Global X S&amp;P 500 Covered Call ETF</name>
        <lei>549300704SF6IBNM0N88</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>37954Y475</cusip>
        <identifiers>
          <isin value="US37954Y4750"/>
        </identifiers>
        <balance>10100</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>398344</valUSD>
        <pctVal>0.00993</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Innovator US Equity Accelerated ETF - Quarterly</name>
        <lei>549300UWCYU2J9ZS4518</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>45783Y103</cusip>
        <identifiers>
          <isin value="US45783Y1038"/>
        </identifiers>
        <balance>25000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>739875</valUSD>
        <pctVal>0.01844</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Invesco QQQ Trust Series 1</name>
        <lei>549300VY6FEJBCIMET58</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>46090E103</cusip>
        <identifiers>
          <isin value="US46090E1038"/>
        </identifiers>
        <balance>8173</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>3347006.96</valUSD>
        <pctVal>0.08342</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares U.S. Technology ETF</name>
        <lei>5493003RR427QZS78C77</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>464287721</cusip>
        <identifiers>
          <isin value="US4642877215"/>
        </identifiers>
        <balance>17314</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2125293.5</valUSD>
        <pctVal>0.05297</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ProShares Ultra Dow30</name>
        <lei>7N3EJP31CN65H4G3UK92</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>74347R305</cusip>
        <identifiers>
          <isin value="US74347R3057"/>
        </identifiers>
        <balance>12200</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>966362</valUSD>
        <pctVal>0.02409</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ProShares Ultra QQQ</name>
        <lei>BB4MTKSM4GAKW287HJ60</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>74347R206</cusip>
        <identifiers>
          <isin value="US74347R2067"/>
        </identifiers>
        <balance>45460</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>3454960</valUSD>
        <pctVal>0.08611</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vanguard Total Stock Market ETF</name>
        <lei>N/A</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>922908769</cusip>
        <identifiers>
          <isin value="US9229087690"/>
        </identifiers>
        <balance>24300</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>5764446</valUSD>
        <pctVal>0.14368</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>WisdomTree PutWrite Strategy</name>
        <lei>549300YBII3KW6EABE35</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>97717X560</cusip>
        <identifiers>
          <isin value="US97717X5602"/>
        </identifiers>
        <balance>12600</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>399420</valUSD>
        <pctVal>0.00996</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Direxion HCM Tactical Enhanced US ETF</name>
        <lei>5493000GOMEINREXLY52</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>25461A726</cusip>
        <identifiers>
          <isin value="US25461A7265"/>
        </identifiers>
        <balance>108431</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2887517.53</valUSD>
        <pctVal>0.07197</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>HCM Defender 100 Index ETF</name>
        <lei>5493009UVJWVJ3E1I465</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>66538R748</cusip>
        <identifiers>
          <isin value="US66538R7483"/>
        </identifiers>
        <balance>52734</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2650990.91</valUSD>
        <pctVal>0.06607</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>HCM Defender 500 Index ETF</name>
        <lei>549300EDDAIZBPZQ5F37</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>66538R730</cusip>
        <identifiers>
          <isin value="US66538R7301"/>
        </identifiers>
        <balance>61751</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2541498.26</valUSD>
        <pctVal>0.06335</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>First Trust Exchange-Traded Fund III-First Trust Long/Short Equity ETF</name>
        <lei>549300751U9S8UPOSL94</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>33739P103</cusip>
        <identifiers>
          <isin value="US33739P1030"/>
        </identifiers>
        <balance>26600</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1496516</valUSD>
        <pctVal>0.0373</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Fidelity Government Portfolio</name>
        <lei>549300FQDFZGLUOCWN51</lei>
        <title>MONEY MARKET FUND</title>
        <cusip>316175108</cusip>
        <identifiers>
          <isin value="US3161751082"/>
        </identifiers>
        <balance>4411897.12</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>4411897.12</valUSD>
        <pctVal>0.10996</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>First American Treasury Obligations Fund</name>
        <lei>549300UU586IAH2B8H03</lei>
        <title>MONEY MARKET FUND</title>
        <cusip>31846V328</cusip>
        <identifiers>
          <isin value="US31846V3289"/>
        </identifiers>
        <balance>6190442.55</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>6190442.55</valUSD>
        <pctVal>0.15429</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <signature>
      <ncom:dateSigned>2024-02-28</ncom:dateSigned>
      <ncom:nameOfApplicant>Tactical Investment Series Trust</ncom:nameOfApplicant>
      <ncom:signature>/s/ Larry E. Beaver, Jr.</ncom:signature>
      <ncom:signerName>Larry E. Beaver, Jr.</ncom:signerName>
      <ncom:title>Treasurer and Principal Financial Officer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
