<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
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    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001843263</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>


      <seriesClassInfo>
        <seriesId>S000071479</seriesId>
        <classId>C000226698</classId>
        <classId>C000226697</classId>
      </seriesClassInfo>


    </filerInfo>
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      <regName>Tactical Investment Series Trust</regName>
      <regFileNumber>811-23631</regFileNumber>
      <regCik>0001843263</regCik>
      <regLei>5493006GAMP0G864BR33</regLei>
      <regStreet1>11726 Seven Gables Road</regStreet1>
      <regCity>Cincinnati</regCity>
      <regStateConditional regCountry="US" regState="US-OH"/>
      <regZipOrPostalCode>45249</regZipOrPostalCode>
      <regPhone>631-470-2688</regPhone>
      <seriesName>TFA Quantitative Fund</seriesName>
      <seriesId>S000071479</seriesId>
      <seriesLei>54930029OKZ3T8LPU649</seriesLei>
      <repPdEnd>2023-12-31</repPdEnd>
      <repPdDate>2023-09-30</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
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      <totLiabs>85829.57</totLiabs>
      <netAssets>27032021.06</netAssets>
      <assetsAttrMiscSec>0</assetsAttrMiscSec>
      <assetsInvested>0</assetsInvested>
      <amtPayOneYrBanksBorr>0</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0</amtPayAftOneYrOther>
      <delayDeliv>0</delayDeliv>
      <standByCommit>0</standByCommit>
      <liquidPref>0</liquidPref>
      <cshNotRptdInCorD>386269.41</cshNotRptdInCorD>
      <curMetrics>
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      <creditSprdRiskNonInvstGrade period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
      <isNonCashCollateral>N</isNonCashCollateral>
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        <monthlyTotReturns>
          <monthlyTotReturn classId="C000226698" rtn1="N/A" rtn2="N/A" rtn3="N/A"/>
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        <othMon2 netRealizedGain="51895.57" netUnrealizedAppr="-769626.51"/>
        <othMon3 netRealizedGain="-640.5" netUnrealizedAppr="-1718815.39"/>
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      <mon3Flow redemption="1209544.88" reinvestment="0" sales="157226.22"/>

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        <name>Global X S&amp;P 500 Covered Call ETF</name>
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        <name>Vanguard Total Stock Market ETF</name>
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      <invstOrSec>
        <name>Direxion HCM Tactical Enhanced US ETF</name>
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      <invstOrSec>
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        <name>First American Treasury Obligations Fund</name>
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        <assetCat>STIV</assetCat>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      </invstOrSec>
    </invstOrSecs>
    <signature>
      <ncom:dateSigned>2023-11-28</ncom:dateSigned>
      <ncom:nameOfApplicant>Tactical Investment Series Trust</ncom:nameOfApplicant>
      <ncom:signature>/s/ Larry E. Beaver, Jr.</ncom:signature>
      <ncom:signerName>Larry E. Beaver, Jr.</ncom:signerName>
      <ncom:title>Treasurer and Principal Financial Officer</ncom:title>
    </signature>
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  <documents>XXXX</documents>
</edgarSubmission>
