<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <headerData>
    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001843263</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>


      <seriesClassInfo>
        <seriesId>S000071480</seriesId>
        <classId>C000226700</classId>
        <classId>C000226699</classId>
      </seriesClassInfo>


    </filerInfo>
  </headerData>
  <formData>
    <genInfo>
      <regName>Tactical Investment Series Trust</regName>
      <regFileNumber>811-23631</regFileNumber>
      <regCik>0001843263</regCik>
      <regLei>5493006GAMP0G864BR33</regLei>
      <regStreet1>11726 Seven Gables Road</regStreet1>
      <regCity>Cincinnati</regCity>
      <regStateConditional regCountry="US" regState="US-OH"/>
      <regZipOrPostalCode>45249</regZipOrPostalCode>
      <regPhone>631-470-2688</regPhone>
      <seriesName>TFA Tactical Income Fund</seriesName>
      <seriesId>S000071480</seriesId>
      <seriesLei>5493008TLLZEF5IJWY68</seriesLei>
      <repPdEnd>2023-12-31</repPdEnd>
      <repPdDate>2023-09-30</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
      <totAssets>43740355.57</totAssets>
      <totLiabs>3139137.55</totLiabs>
      <netAssets>40601218.02</netAssets>
      <assetsAttrMiscSec>0</assetsAttrMiscSec>
      <assetsInvested>0</assetsInvested>
      <amtPayOneYrBanksBorr>0</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0</amtPayAftOneYrOther>
      <delayDeliv>2986291.67</delayDeliv>
      <standByCommit>0</standByCommit>
      <liquidPref>0</liquidPref>
      <cshNotRptdInCorD>4007776.44</cshNotRptdInCorD>
      <curMetrics>
        <curMetric>
          <curCd>USD</curCd>
          <intrstRtRiskdv01 period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
          <intrstRtRiskdv100 period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
        </curMetric>
      </curMetrics>
      <creditSprdRiskInvstGrade period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
      <creditSprdRiskNonInvstGrade period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0"/>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000226700" rtn1="0.24" rtn2="-0.59" rtn3="N/A"/>
          <monthlyTotReturn classId="C000226699" rtn1="0.35" rtn2="-1.88" rtn3="-1.92"/>
        </monthlyTotReturns>
        <othMon1 netRealizedGain="-84399.05" netUnrealizedAppr="144748.85"/>
        <othMon2 netRealizedGain="-790178.07" netUnrealizedAppr="-132157.91"/>
        <othMon3 netRealizedGain="-312620.7" netUnrealizedAppr="-689379"/>
      </returnInfo>
      <mon1Flow redemption="1487305.72" reinvestment="0" sales="871369.61"/>
      <mon2Flow redemption="3292912.93" reinvestment="0" sales="879363.83"/>
      <mon3Flow redemption="1800825.66" reinvestment="0" sales="296811.95"/>

    </fundInfo>
    <invstOrSecs>
      <invstOrSec>
        <name>iShares Mortgage Real Estate ETF</name>
        <lei>549300WLIJWYXGL2J018</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>46435G342</cusip>
        <identifiers>
          <isin value="US46435G3424"/>
        </identifiers>
        <balance>81000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1809540</valUSD>
        <pctVal>0.04457</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Virtus Private Credit Strategy ETF</name>
        <lei>549300CDY5S895RWHU42</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>26923G798</cusip>
        <identifiers>
          <isin value="US26923G7988"/>
        </identifiers>
        <balance>29500</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>633660</valUSD>
        <pctVal>0.01561</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Direxion Daily 20 Year Plus Treasury Bull 3x Shares</name>
        <lei>HIB1RJORN6PDA2VE6Q12</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>25459W540</cusip>
        <identifiers>
          <isin value="US25459W5408"/>
        </identifiers>
        <balance>555340</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2676738.8</valUSD>
        <pctVal>0.06593</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>First Trust Senior Loan ETF</name>
        <lei>N/A</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>33738D309</cusip>
        <identifiers>
          <isin value="US33738D3098"/>
        </identifiers>
        <balance>31602</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1445475.48</valUSD>
        <pctVal>0.0356</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>FolioBeyond Alternative Income and Interest Rate Hedge ETF</name>
        <lei>549300JTP3K4NOX5GH17</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>886364637</cusip>
        <identifiers>
          <isin value="US8863646373"/>
        </identifiers>
        <balance>79900</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2668500.2</valUSD>
        <pctVal>0.06572</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Invesco Emerging Markets Sovereign Debt ETF</name>
        <lei>549300VQ5VGUIVZMPZ95</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>46138E784</cusip>
        <identifiers>
          <isin value="US46138E7849"/>
        </identifiers>
        <balance>47100</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>860517</valUSD>
        <pctVal>0.02119</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares 0-5 Year TIPS Bond ETF</name>
        <lei>549300GIWSEGOQLZR212</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>46429B747</cusip>
        <identifiers>
          <isin value="US46429B7477"/>
        </identifiers>
        <balance>18000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1744560</valUSD>
        <pctVal>0.04297</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares 10-20 Year Treasury Bond ETF</name>
        <lei>549300MH5J7BUV0VS287</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>464288653</cusip>
        <identifiers>
          <isin value="US4642886539"/>
        </identifiers>
        <balance>1000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>98470</valUSD>
        <pctVal>0.00243</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares 7-10 Year Treasury Bond ETF</name>
        <lei>549300XS1PPZG92M8L16</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>464287440</cusip>
        <identifiers>
          <isin value="US4642874402"/>
        </identifiers>
        <balance>10100</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>925059</valUSD>
        <pctVal>0.02278</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares CMBS ETF</name>
        <lei>5493008QF1WFU4PEB467</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>46429B366</cusip>
        <identifiers>
          <isin value="US46429B3666"/>
        </identifiers>
        <balance>30600</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1378224</valUSD>
        <pctVal>0.03395</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares Core U.S. Aggregate Bond ETF</name>
        <lei>549300F7VSXTKBGHJE47</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>464287226</cusip>
        <identifiers>
          <isin value="US4642872265"/>
        </identifiers>
        <balance>9000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>846360</valUSD>
        <pctVal>0.02085</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares Floating Rate Bond ETF</name>
        <lei>549300G2T0MQ0NCEG317</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>46429B655</cusip>
        <identifiers>
          <isin value="US46429B6552"/>
        </identifiers>
        <balance>28410</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1445784.9</valUSD>
        <pctVal>0.03561</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares iBoxx High Yield Corporate Bond ETF</name>
        <lei>N/A</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>464288513</cusip>
        <identifiers>
          <isin value="US4642885135"/>
        </identifiers>
        <balance>24300</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1791396</valUSD>
        <pctVal>0.04412</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Invesco Senior Loan ETF</name>
        <lei>549300SIHU6DQELRNG59</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>46138G508</cusip>
        <identifiers>
          <isin value="US46138G5080"/>
        </identifiers>
        <balance>130400</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2737096</valUSD>
        <pctVal>0.06741</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ProShares High Yield-Interest Rate Hedged ETF</name>
        <lei>549300FV6XUZGOJNSR91</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>74348A541</cusip>
        <identifiers>
          <isin value="US74348A5415"/>
        </identifiers>
        <balance>13166</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>809972.32</valUSD>
        <pctVal>0.01995</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ProShares Short 20+ Year Treasury</name>
        <lei>MNF315G62CW700KJGX62</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>74347X849</cusip>
        <identifiers>
          <isin value="US74347X8496"/>
        </identifiers>
        <balance>57269</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1440315.35</valUSD>
        <pctVal>0.03547</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>SPDR Bloomberg 1-3 Month T-Bill ETF</name>
        <lei>549300GQCVCME1YJ6B50</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>78468R663</cusip>
        <identifiers>
          <isin value="US78468R6633"/>
        </identifiers>
        <balance>87400</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8025068</valUSD>
        <pctVal>0.19766</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>SPDR Gold Shares</name>
        <lei>N/A</lei>
        <title>EXCHANGE-TRADED FUND</title>
        <cusip>78463V107</cusip>
        <identifiers>
          <isin value="US78463V1070"/>
        </identifiers>
        <balance>9600</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1645920</valUSD>
        <pctVal>0.04054</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Fidelity Government Portfolio</name>
        <lei>549300FQDFZGLUOCWN51</lei>
        <title>MONEY MARKET FUND</title>
        <cusip>316175108</cusip>
        <identifiers>
          <isin value="US3161751082"/>
        </identifiers>
        <balance>402025.17</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>402025.17</valUSD>
        <pctVal>0.0099</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>First American Treasury Obligations Fund</name>
        <lei>549300UU586IAH2B8H03</lei>
        <title>MONEY MARKET FUND</title>
        <cusip>31846V328</cusip>
        <identifiers>
          <isin value="US31846V3289"/>
        </identifiers>
        <balance>6347896.91</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>6347896.91</valUSD>
        <pctVal>0.15635</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <signature>
      <ncom:dateSigned>2023-11-28</ncom:dateSigned>
      <ncom:nameOfApplicant>Tactical Investment Series Trust</ncom:nameOfApplicant>
      <ncom:signature>/s/ Larry E. Beaver, Jr.</ncom:signature>
      <ncom:signerName>Larry E. Beaver, Jr.</ncom:signerName>
      <ncom:title>Treasurer and Principal Financial Officer</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
