<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2024-07-31</periodOfReport>

    <notSubjectToSection16>true</notSubjectToSection16>

    <issuer>
        <issuerCik>0001838821</issuerCik>
        <issuerName>ARYA Sciences Acquisition Corp IV</issuerName>
        <issuerTradingSymbol>ARYD</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001678736</rptOwnerCik>
            <rptOwnerName>Poukalov Konstantin</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O ARYA SCIENCES ACQUISITION CORP IV</rptOwnerStreet1>
            <rptOwnerStreet2>51 ASTOR PLACE, 10TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10003</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>true</isOfficer>
            <officerTitle>Chief Business Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

    <ownerSignature>
        <signatureName>/s/ Samuel M. Cohn, attorney-in-fact</signatureName>
        <signatureDate>2024-07-31</signatureDate>
    </ownerSignature>
</ownershipDocument>
