144 0001837756 XXXXXXXX LIVE 0000834365 BIOLIFE SOLUTIONS INC 001-36362 3303 Monte Villa Parkway, Suite 310 Bothell WA 98021 4254021400 SARAH AEBERSOLD Officer Common Morgan Stanley Smith Barney LLC Executive Financial Services

1 New York Plaza 8th Floor New York NY 10004

7324 194964.88 47000000 03/12/2025 NASDAQ Common 03/01/2023 RSU/RSA/PSU Issuer N 7324 03/01/2023 N/A N SARAH AEBERSOLD

3303 Monte Villa Parkway, Suite 310 Bothell WA 98021

Common 03/03/2025 284 7230.64 SARAH AEBERSOLD

3303 Monte Villa Parkway, Suite 310 Bothell WA 98021

Common 02/12/2025 53 1406.62 SARAH AEBERSOLD

3303 Monte Villa Parkway, Suite 310 Bothell WA 98021

Common 01/06/2025 240 6484.80 The securities to be sold were acquired upon the vesting of RSUs, RSAs, and PSUs during the period of 03/01/2023 through 10/20/2023. 03/12/2025 12/11/2024 /s/ Sarah Aebersold