Exhibit 15.3

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statements on Form F-3 (No. 333-269285) and Form S-8 (Nos. 333-264181 and 333-254844) of Olink Holding AB (publ)of our report datedMarch 17, 2022 relating to the financial statements which appears in this Form 20-F.

/s/Öhrlings PricewaterhouseCoopers AB

Stockholm, Sweden

March 27, 2023