<?xml version="1.0" encoding="UTF-8"?>
<edgarSubmission xsi:schemaLocation="http://www.sec.gov/edgar/thirteenffiler eis_13F_Filer.xsd" xmlns="http://www.sec.gov/edgar/thirteenffiler" xmlns:ns1="http://www.sec.gov/edgar/common" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <schemaVersion>X0202</schemaVersion>
  <headerData>
    <submissionType>13F-HR</submissionType>
    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <flags>
        <confirmingCopyFlag>false</confirmingCopyFlag>
        <returnCopyFlag>false</returnCopyFlag>
        <overrideInternetFlag>false</overrideInternetFlag>
      </flags>
      <filer>
        <credentials>
          <cik>0001832749</cik>
          <ccc>XXXXXXXX</ccc>
        </credentials>
      </filer>
      <periodOfReport>12-31-2025</periodOfReport>
    </filerInfo>
  </headerData>
  <formData>
    <coverPage>
      <reportCalendarOrQuarter>12-31-2025</reportCalendarOrQuarter>
      <isAmendment>false</isAmendment>
      <filingManager>
        <name>72 Investment Holdings, LLC</name>
        <address>
          <ns1:street1>72 CUMMINGS POINT ROAD</ns1:street1>
          <ns1:city>STAMFORD</ns1:city>
          <ns1:stateOrCountry>CT</ns1:stateOrCountry>
          <ns1:zipCode>06902</ns1:zipCode>
        </address>
      </filingManager>
      <reportType>13F HOLDINGS REPORT</reportType>
      <form13FFileNumber>028-20409</form13FFileNumber>
      <provideInfoForInstruction5>Y</provideInfoForInstruction5>
      <additionalInformation>The Reporting Manager, 72 Investment Holdings, LLC, is under common control with Point72 Asset Management, L.P. (Form 13F File Number 028-16256), CPV Partners, LLC (Form 13F File Number 028-19347), and Point72 Private Investments, LLC (Form 13F File Number 028-23092) each of which separately file Form 13F-HR. * Positions that are exempt from reporting under Special Instruction 10 to the Form 13F are excluded from this report. However, positions that would not be exempt when aggregated with positions of the same security over which each of the foregoing managers have investment discretion are listed herein.</additionalInformation>
    </coverPage>
    <signatureBlock>
      <name>Jason M. Colombo</name>
      <title>Authorized Person</title>
      <phone>203-890-2000</phone>
      <signature>/s/ Jason M. Colombo</signature>
      <city>Stamford</city>
      <stateOrCountry>CT</stateOrCountry>
      <signatureDate>02-17-2026</signatureDate>
    </signatureBlock>
    <summaryPage>
      <otherIncludedManagersCount>0</otherIncludedManagersCount>
      <tableEntryTotal>5</tableEntryTotal>
      <tableValueTotal>50574739</tableValueTotal>
    </summaryPage>
  </formData>
</edgarSubmission>
