<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2020-12-14</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001830180</issuerCik>
        <issuerName>Marquee Raine Acquisition Corp.</issuerName>
        <issuerTradingSymbol>MRAC</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001292875</rptOwnerCik>
            <rptOwnerName>Freston Thomas E</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O MARQUEE RAINE ACQUISITION CORP.</rptOwnerStreet1>
            <rptOwnerStreet2>65 EAST 55TH STREET, 24TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Class B Ordinary Shares, par value $0.0001</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F1"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F1"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Class A Ordinary Shares, par value $0.0001</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>25000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The reporting person owns 25,000 Class B ordinary shares, par value $0.0001 per share (&quot;Class B Ordinary Shares&quot;), which are convertible into Class A Ordinary Shares, par value $0.0001 per share, as described under the heading &quot;Description of Securities-Founder Shares&quot; in the Issuer's registration statement on Form S-1 (File No. 333-250997). The Class B Ordinary Shares have no expiration date.</footnote>
    </footnotes>

    <remarks>Exhibit 24.1 Power of Attorney</remarks>

    <ownerSignature>
        <signatureName>/s/ Alfred Chianese, Attorney-in-Fact for Thomas Freston</signatureName>
        <signatureDate>2020-12-14</signatureDate>
    </ownerSignature>
</ownershipDocument>
