X0407 4 2023-06-07 0 0001827087 Vigil Neuroscience, Inc. VIGL 0001826967 Vitorovic Stefan VIGIL NEUROSCIENCE, INC. 100 FORGE ROAD, SUITE 700 WATERTOWN MA 02472 1 0 0 0 0 Common Stock 5004393 I By Vida Ventures III, L.P. Common Stock 11536 I By Vida Ventures III-A, L.P. Stock Option Grant (Right to Buy) 10.00 2023-06-07 4 A 0 13928 0.00 A 2033-06-07 Common Stock 13928 13928 D The shares are held directly by Vida Ventures III, L.P. ("Vida III"). Vida Ventures GP III, L.L.C. ("Vida III GP") is the general partner of Vida III. The Reporting Person is a managing member of Vida III GP and disclaims beneficial ownership of the shares held directly by Vida III, except to the extent of his pecuniary interest therein, if any. The shares are held directly by Vida Ventures III-A, L.P. ("Vida III-A"). Vida III GP is the general partner of Vida III-A. The Reporting Person is a managing member of Vida III GP and disclaims beneficial ownership of the shares held directly by Vida III-A, except to the extent of his pecuniary interest therein, if any. The shares subject to this option shall vest and become exercisable upon the earlier to occur of (i) June 7, 2024 and (ii) the next annual meeting of the Issuer's stockholders. Exhibit 24 - Power of Attorney /s/ Christopher Verni, Attorney-in-Fact 2023-06-09