X0306 4 2023-03-01 0 0001827087 Vigil Neuroscience, Inc. VIGL 0001517790 Bruhn Suzanne Louise VIGIL NEUROSCIENCE, INC. 100 FORGE ROAD, SUITE 700 WATERTOWN MA 02472 1 0 0 0 Stock Option Grant (Right to Buy) 11.87 2023-03-01 4 A 0 6235 7.618 A 2033-03-01 Common Stock 6235 6235 D This option was awarded to the reporting person pursuant to the Issuer's Non-Employee Director Compensation Policy in lieu of cash compensation. The shares subject to this option shall vest and become exercisable in four equal quarterly installments on the last day of each calendar quarter, with the first installment vesting on March 31, 2023. /s/ Christopher Verni, Attorney-in-Fact 2023-03-03