X0306 4 2022-05-02 0 0001827087 Vigil Neuroscience, Inc. VIGL 0001279281 Magovcevic-Liebisch Ivana VIGIL NEUROSCIENCE, INC. 1 BROADWAY, 7TH FLOOR, SUITE 07-300 CAMBRIDGE MA 02142 1 1 0 0 President and CEO Common Stock 2022-05-02 4 P 0 4000 3.6097 A 202687 D Common Stock 2022-05-03 4 P 0 1000 3.9402 A 203687 D The price reported in Column 4 is a weighted average price. The shares were purchased in multiple transactions at prices ranging from $3.27 to $3.79, inclusive. The Reporting Person undertakes to provide to the Issuer, any security holder of the Issuer or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares purchased at each separate price at which the transactions were effected. The price reported in Column 4 is a weighted average price. The shares were purchased in multiple transactions at prices ranging from $3.89 to $4.00, inclusive. The Reporting Person undertakes to provide to the Issuer, any security holder of the Issuer or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares purchased at each separate price at which the transactions were effected. /s/ Jennifer Ziolkowski, Attorney-in-Fact 2022-05-04