X0306 4 2022-03-01 0 0001827087 Vigil Neuroscience, Inc. VIGL 0001326530 Blanchard Cheryl R VIGIL NEUROSCIENCE, INC. 1 BROADWAY, 7TH FLOOR, SUITE 07-300 CAMBRIDGE MA 02142 1 0 0 0 Stock Option Grant (Right to Buy) 16.13 2022-03-01 4 A 0 5017 9.63 A 2032-03-01 Common Stock 5017 5017 D This option was awarded to the reporting person pursuant to the Issuer's Non-Employee Director Compensation Policy in lieu of cash compensation. The shares subject to this option shall vest and become exercisable in four equal quarterly installments on the last day of each calendar quarter, with the first installment vesting on March 31, 2022. /s/ Jennifer Ziolkowski, Attorney-in-Fact 2022-03-03