X0206 3 2022-01-06 0 0001827087 Vigil Neuroscience, Inc. VIGL 0001826967 Vitorovic Stefan VIGIL NEUROSCIENCE, INC. 1 BROADWAY, 7TH FLOOR, SUITE 07-300 CAMBRIDGE MA 02142 1 0 0 0 Series B Preferred Stock Common Stock 3333245 I By Vida Ventures III, L.P. Series B Preferred Stock Common Stock 7684 I By Vida Ventures III-A, L.P. These shares of Series B Preferred Stock are convertible into Common Stock on a one-for-2.7732 basis at the option of the holder, and will automatically convert into the number of shares shown in Column 3 immediately prior to the closing of the Issuer's initial public offering. The Series B Preferred Stock has no expiration date. The shares are held directly by Vida Ventures III, L.P. ("Vida III"). Vida Ventures GP III, L.L.C. ("Vida III GP") is the general partner of of Vida III. The Reporting Person is a managing member of Vida III GP and disclaims beneficial ownership of the shares held directly by Vida III, except to the extent of his pecuniary interest therein, if any. The shares are held directly by Vida Ventures III-A, L.P. ("Vida III-A"). Vida III GP is the general partner of of Vida III-A. The Reporting Person is a managing member of Vida III GP and disclaims beneficial ownership of the shares held directly by Vida III-A, except to the extent of his pecuniary interest therein, if any. Exhibit 24 - Power of Attorney /s/ Jennifer Ziolkowski, Attorney-in-Fact 2022-01-06