X0206 3 2022-01-06 0 0001827087 Vigil Neuroscience, Inc. VIGL 0001592380 Thorp Clay VIGIL NEUROSCIENCE, INC. 1 BROADWAY, 7TH FLOOR, SUITE 07-300 CAMBRIDGE MA 02142 1 0 0 0 Series A Preferred Stock Common Stock 920243 I By Hatteras Venture Partners VI, L.P. Series B Preferred Stock Common Stock 334092 I By Hatteras Venture Partners VI, L.P. These shares of Series A Preferred Stock and Series B Preferred Stock (collectively, the "Preferred Stock") are convertible into Common Stock on a one-for-2.7732 basis at the option of the holder, and will automatically convert into the number of shares shown in Column 3 immediately prior to the closing of the Issuer's initial public offering. The Preferred Stock has no expiration date. The shares are held directly by Hatteras Venture Partners VI, L.P. ("Hatteras VI"). Hatteras Venture Advisors VI, LLC ("HVA VI") is the general partner of Hatteras VI. The Reporting Person is a managing member of HVA VI and disclaims beneficial ownership of the shares held by Hatteras VI except to the extent of his pecuniary interest therein, if any. Exhibit 24 - Power of Attorney /s/ Jennifer Ziolkowski, Attorney-in-Fact 2022-01-06