X0206 3 2022-01-06 0 0001827087 Vigil Neuroscience, Inc. VIGL 0001727410 Ziolkowski Jennifer Lynn VIGIL NEUROSCIENCE, INC. 1 BROADWAY, 7TH FLOOR, SUITE 07-300 CAMBRIDGE MA 02142 0 1 0 0 Chief Financial Officer Stock Option Grant (Right to Buy) 3.78 2031-06-03 Common Stock 119923 D Stock Option Grant (Right to Buy) 3.78 2031-06-03 Common Stock 49968 D Stock Option Grant (Right to Buy) 6.02 2031-08-18 Common Stock 96051 D Stock Option Grant (Right to Buy) 9.57 2031-10-13 Common Stock 21635 D 25% of the shares subject to this option shall vest and become exercisable on March 1, 2022, with the remainder vesting in 36 equal monthly installments thereafter. 25% of the shares subject to this option shall vest and become exercisable on June 1, 2022, with the remainder vesting in 36 equal monthly installments thereafter. The shares subject to this option vest and become exercisable in 48 equal monthly installments, with the first installment vested September 13, 2021. The shares subject to this option vest and become exercisable in 48 equal monthly installments, with the first installment vested November 14, 2021. Exhibit 24 - Power of Attorney /s/ Jennifer Ziolkowski 2022-01-06