<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2023-10-05</periodOfReport>

    <issuer>
        <issuerCik>0001821586</issuerCik>
        <issuerName>MoonLake Immunotherapeutics</issuerName>
        <issuerTradingSymbol>MLTX</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001599214</rptOwnerCik>
            <rptOwnerName>Chen Bihua</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O CORMORANT ASSET MANAGEMENT, LP</rptOwnerStreet1>
            <rptOwnerStreet2>200 CLARENDON STREET, 52ND FLOOR</rptOwnerStreet2>
            <rptOwnerCity>BOSTON</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02116</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001583977</rptOwnerCik>
            <rptOwnerName>Cormorant Asset Management, LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>200 CLARENDON STREET</rptOwnerStreet1>
            <rptOwnerStreet2>52ND FLOOR</rptOwnerStreet2>
            <rptOwnerCity>BOSTON</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02116</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001618442</rptOwnerCik>
            <rptOwnerName>Cormorant Global Healthcare Master Fund, LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>200 CLARENDON STREET</rptOwnerStreet1>
            <rptOwnerStreet2>52ND FLOOR</rptOwnerStreet2>
            <rptOwnerCity>BOSTON</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02116</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001747677</rptOwnerCik>
            <rptOwnerName>Cormorant Private Healthcare Fund II, LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>200 CLARENDON STREET, 52ND FLOOR</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>BOSTON</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02116</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001817320</rptOwnerCik>
            <rptOwnerName>Cormorant Private Healthcare Fund III LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>200 CLARENDON STREET</rptOwnerStreet1>
            <rptOwnerStreet2>52ND FLOOR</rptOwnerStreet2>
            <rptOwnerCity>BOSTON</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02116</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001871297</rptOwnerCik>
            <rptOwnerName>Cormorant Private Healthcare Fund IV LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>200 CLARENDON STREET</rptOwnerStreet1>
            <rptOwnerStreet2>52ND FLOOR</rptOwnerStreet2>
            <rptOwnerCity>BOSTON</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02116</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A Ordinary Shares</value>
            </securityTitle>
            <transactionDate>
                <value>2023-10-05</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>P</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>58839</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>59.8973</value>
                    <footnoteId id="F1"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>8494151</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See Footnotes</value>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                    <footnoteId id="F4"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Represents the weighted average sale price of Class A Ordinary Shares (&quot;Shares&quot;) purchased in a series of open market transactions on the transaction date at prices ranging from $59.78 to $59.90 per Share. The Reporting Persons undertake to provide, upon request by the staff of the Securities and Exchange Commission, the issuer, or a security holder of the issuer, full information regarding the number of Shares purchased at each price. All of these Shares were purchased by the Master Fund (as defined below).</footnote>
        <footnote id="F2">Information reported herein relates to Shares owned by Cormorant Global Healthcare Master Fund, LP (the &quot;Master Fund&quot;), Cormorant Private Healthcare Fund II, LP (&quot;Fund II&quot;), Cormorant Private Healthcare Fund III, LP (&quot;Fund III&quot;), Cormorant Private Healthcare Fund IV, LP (&quot;Fund IV&quot;) and a managed account (the &quot;Account&quot; and together with the Master Fund, Fund II, Fund III and Fund IV, the &quot;Funds&quot;)). Cormorant Asset Management, LP (&quot;Cormorant&quot;) serves as the investment manager of the Funds. Cormorant Global Healthcare GP, LLC (&quot;GP LLC&quot;), Cormorant Private Healthcare GP II, LLC (&quot;GP II&quot;), Cormorant Private Healthcare GP III, LLC (&quot;GP III&quot;) and Cormorant Private Healthcare GP IV, LLC (&quot;GP IV&quot;) serve as General Partner of the Master Fund, Fund II, Fund III and Fund IV, respectively. Bihua Chen serves as manager of Cormorant, GP LLC, GP II, GP III and GP IV.</footnote>
        <footnote id="F3">Each of the Reporting Persons disclaims beneficial ownership of the Shares reported herein except to the extent of its or her pecuniary interest therein, and the filing of this Form 4 shall not be construed as an admission that any of the Reporting Persons is the beneficial owner of any such ordinary shares for purposes of Section 16(a) of the Securities Exchange Act of 1934 or for any other purpose.</footnote>
        <footnote id="F4">Represents (i) 2,712,779 Shares beneficially owned by the Master Fund, (ii) 1,802,737 Shares beneficially owned by Fund II, (iii) 2,453,879 Shares beneficially owned by Fund III, (iv) 1,500,000 Shares beneficially owned by Fund IV, and (v) 24,756 shares beneficially owned by the Account.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Bihua Chen</signatureName>
        <signatureDate>2023-10-10</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ CORMORANT ASSET MANAGEMENT, LP By: Cormorant Asset Management GP, LLC, its General Partner, By: Bihua Chen, Managing Member</signatureName>
        <signatureDate>2023-10-10</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ CORMORANT GLOBAL HEALTHCARE MASTER FUND, LP,
By: Cormorant Global Healthcare GP, LLC, its General Partner
By: Bihua Chen, Managing Member</signatureName>
        <signatureDate>2023-10-10</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ CORMORANT PRIVATE HEALTHCARE FUND II, LP
By: Cormorant Private Healthcare GP II, LLC, its General Partner
By: Bihua Chen, Managing Member</signatureName>
        <signatureDate>2023-10-10</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ CORMORANT PRIVATE HEALTHCARE FUND III, LP
By: Cormorant Private Healthcare GP III, LLC, its General Partner
By: Bihua Chen, Managing Member</signatureName>
        <signatureDate>2023-10-10</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ CORMORANT PRIVATE HEALTHCARE FUND IV, LP
By: Cormorant Private Healthcare GP IV, LLC, its General Partner
By: Bihua Chen, Managing Member</signatureName>
        <signatureDate>2023-10-10</signatureDate>
    </ownerSignature>
</ownershipDocument>
