EXHIBIT 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the following Registration Statements:

1. Registration Statement (Form S-8 No. 333-255061) pertaining to the Gain Therapeutics, Inc.  2020 Omnibus Incentive Plan,

2. Registration Statement (Form S-8 No. 333-266142) pertaining to the Gain Therapeutics, Inc. 2021 Inducement Equity Incentive Plan and the Gain Therapeutics, Inc. 2022 Equity Incentive Plan,

3. Registration Statement (Form S-8 No. 333-272255) pertaining to the Gain Therapeutics, Inc. 2022 Equity Incentive Plan,

4. Registration Statement (Form S-3 No. 333-265061) of Gain Therapeutics, Inc., and

5. Registration Statement (Form S-1 No. 333-276145) of Gain Therapeutics, Inc.;

of our report dated March 26, 2024, with respect to the consolidated financial statements of Gain Therapeutics, Inc., included in this Annual Report (Form 10-K) for the year ended December 31, 2023.

/s/ Ernst & Young AG

Lugano, Switzerland

March 26, 2024