X0306 4 2022-12-31 0 0001818331 Sema4 Holdings Corp. SMFR 0001585017 Ryan Jason C/O SEMA4 HOLDINGS CORP. 333 LUDLOW ST, NORTH TOWER, 8TH FL. STAMFORD CT 06902 1 1 0 0 Executive Chairman Class A Common Stock 2022-12-31 4 M 0 247525 A 263358 D Class A Common Stock 2022-12-31 4 S 0 73732 0.2619 D 189626 D Restricted Stock Unit 2022-12-31 4 M 0 247525 0.00 D Class A Common Stock 247525 0 D Each restricted stock unit ("RSU") represents a contingent right to receive 1 share of the Issuer's Class A Common Stock upon settlement for no consideration. The sale reported on this Form 4 represents shares sold by the Reporting Person to cover tax withholding obligations in connection with the vesting and settlement of RSUs. The sale was to satisfy tax withholding obligations to be funded by a "sell to cover" transaction and does not represent a discretionary transaction by the Reporting Person. 100% of the RSUs vested as of December 31, 2022, subject to the Reporting Person's continued service to the Issuer on such earlier date, or upon a termination of the Reporting Person's service as Executive Chairman of the Board under certain circumstances. /s/ Bridget Brown, Attorney-in-Fact 2023-01-04