SCHEDULE 13D/A 0001104659-23-023247 0001966029 XXXXXXXX LIVE 4 Ordinary Shares, nominal value GBP0.0025 per share underlying American Depositary Shares 11/04/2025 false 0001811115 75973T200 Renalytix plc

2 Leman Street LONDON X0 E1W 9US

PETER TRAPANI 212-805-8110 JEFFERSON RIVER CAPITAL LLC 499 PARK AVENUE, 27TH FLOOR NEW YORK NY 10022 0001966029 N JEFFERSON RIVER CAPITAL LLC b AF N DE 0.00 19644391.00 0.00 19644391.00 19644391.00 N 4.5 IA Ownership percentage calculations are based on 437,018,680 Ordinary Shares outstanding as of November 3, 2025, as indicated by the Issuer's proxy statement dated November 4, 2025. Y The Hamilton E. James 2003 Children's Trust b WC N NY 0.00 19406043.00 0.00 19406043.00 19406043.00 N 4.4 OO Y Hamilton E. James b AF PF N X1 0.00 19644391.00 0.00 19644391.00 19644391.00 N 4.5 IN HC Y David R. James AF N X1 0.00 19406043.00 0.00 19406043.00 19406043.00 N 4.4 IN HC Ordinary Shares, nominal value GBP0.0025 per share underlying American Depositary Shares Renalytix plc 2 Leman Street LONDON X0 E1W 9US This Amendment No. 4 ("Amendment No. 4") amends the statement on Schedule 13D filed with the Securities and Exchange Commission on February 17, 2023, as amended by Amendment No. 1 on March 21, 2024, Amendment No. 2 on October 2, 2024 and Amendment No. 3 on November 6, 2024. This Amendment No. 4 relates to ordinary shares, par value GBP0.0025 (the "Ordinary Shares") of Renalytix plc, a company incorporated in England and Wales (the "Issuer"). The principal executive office of the Issuer is located at 2 Leman Street, London E1W 9US, United Kingdom. The beneficial ownership reported herein reflects Ordinary Shares and the Issuer's American depositary shares (the "ADS"), each representing two Ordinary Shares, held by the Reporting Persons (as defined in the Schedule 13D). Items 7 through 11 and 13 of each of the cover pages of this Schedule 13D are incorporated herein by reference. HEJ is the direct beneficial owner of 238,348 Ordinary Shares in the form of ADS. The percentages reported in this Schedule 13D are calculated based upon 437,018,680 Ordinary Shares outstanding as of November 3, 2025, as indicated by the Issuer's proxy statement dated November 4, 2025. Jefferson River disclaims beneficial ownership over all securities beneficially owned by the Trust and HEJ, other than for the purpose of determining obligations under the Securities Exchange Act of 1934, as amended (the "Exchange Act"), and the filing of this Schedule 13D shall not be deemed an admission that Jefferson River is the beneficial owner of such securities for any other purpose. HEJ disclaims beneficial ownership over all securities beneficially owned by the Trust, other than for the purpose of determining obligations under the Exchange Act, and the filing of this Schedule 13D shall not be deemed an admission that HEJ is the beneficial owner of such securities for any other purpose. DRJ disclaims beneficial ownership over all securities beneficially owned by the Trust, other than for the purpose of determining obligations under the Exchange Act, and the filing of this Schedule 13D shall not be deemed an admission that DRJ is the beneficial owner of such securities for any other purpose. Items 7 through 11 and 13 of each of the cover pages of this Schedule 13D are incorporated herein by reference. HEJ is the direct beneficial owner of 238,348 Ordinary Shares in the form of ADS. The percentages reported in this Schedule 13D are calculated based upon 437,018,680 Ordinary Shares outstanding as of November 3, 2025, as indicated by the Issuer's proxy statement dated November 4, 2025. Jefferson River disclaims beneficial ownership over all securities beneficially owned by the Trust and HEJ, other than for the purpose of determining obligations under the Securities Exchange Act of 1934, as amended (the "Exchange Act"), and the filing of this Schedule 13D shall not be deemed an admission that Jefferson River is the beneficial owner of such securities for any other purpose. HEJ disclaims beneficial ownership over all securities beneficially owned by the Trust, other than for the purpose of determining obligations under the Exchange Act, and the filing of this Schedule 13D shall not be deemed an admission that HEJ is the beneficial owner of such securities for any other purpose. DRJ disclaims beneficial ownership over all securities beneficially owned by the Trust, other than for the purpose of determining obligations under the Exchange Act, and the filing of this Schedule 13D shall not be deemed an admission that DRJ is the beneficial owner of such securities for any other purpose. Each of the Reporting Persons ceased to be the beneficial owner of more than 5% of the Ordinary Shares on November 3, 2025. JEFFERSON RIVER CAPITAL LLC /s/ Peter Trapani 02/23/2026 The Hamilton E. James 2003 Children's Trust /s/ Peter Trapani 02/23/2026 Hamilton E. James /s/ Hamilton E. James 02/23/2026 David R. James /s/ David R. James 02/23/2026