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  <headerData>
    <submissionType>13F-HR</submissionType>
    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <flags>
        <confirmingCopyFlag>false</confirmingCopyFlag>
        <returnCopyFlag>false</returnCopyFlag>
        <overrideInternetFlag>false</overrideInternetFlag>
      </flags>
      <filer>
        <credentials>
          <cik>0001807328</cik>
          <ccc>XXXXXXXX</ccc>
        </credentials>
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      <periodOfReport>09-30-2020</periodOfReport>
    </filerInfo>
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    <coverPage>
      <reportCalendarOrQuarter>09-30-2020</reportCalendarOrQuarter>
      <isAmendment>false</isAmendment>
      <filingManager>
        <name>LIBERTY ONE INVESTMENT MANAGEMENT, LLC</name>
        <address>
          <ns1:street1>1509 N. MILWAUKEE AVE.</ns1:street1>
          <ns1:city>LIBERTYVILLE</ns1:city>
          <ns1:stateOrCountry>IL</ns1:stateOrCountry>
          <ns1:zipCode>60048</ns1:zipCode>
        </address>
      </filingManager>
      <reportType>13F HOLDINGS REPORT</reportType>
      <form13FFileNumber>028-20219</form13FFileNumber>
      <provideInfoForInstruction5>N</provideInfoForInstruction5>
    </coverPage>
    <signatureBlock>
      <name>Nick Ng</name>
      <title>Portfolio Manager</title>
      <phone>847-680-9255</phone>
      <signature>/s/ Nick Ng</signature>
      <city>Libertyville</city>
      <stateOrCountry>IL</stateOrCountry>
      <signatureDate>11-02-2020</signatureDate>
    </signatureBlock>
    <summaryPage>
      <otherIncludedManagersCount>0</otherIncludedManagersCount>
      <tableEntryTotal>135</tableEntryTotal>
      <tableValueTotal>488945</tableValueTotal>
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