144 0001807214 XXXXXXXX LIVE 0001426800 Assembly Biosciences, Inc. 001-35005 331 Oyster Point Blvd. Fourth Floor South San Francisco CA 94080 833-509-4583 Jason Okazaki Officer Director Common Stock Morgan Stanley Smith Barney LLC

1 New York Plaza 38th Floor New York NY 10004

11827 12866.59 52121309 08/02/2023 Nasdaq Common Stock 08/01/2023 Restricted Stock Unit Release Assembly Biosciences, Inc. N 11827 08/02/2023 N/A N Jason A. Okazaki

c/o 331 Oyster Point Blvd. Fourth Floor South San Francisco CA 94080

Common Stock 05/22/2023 6561 6825.41 08/02/2023 /s/ Jason A. Okazaki