X0202 SCHEDULE 13G 0001646799 XXXXXXXX LIVE Common Stock, par value $0.0001 per share 04/27/2026 0001807166 Amesite Inc. 031094204 607 Shelby Street Suite 700 PMB 214 Detroit MI 48226 Rule 13d-1(c) Mitchell P. Kopin X1 0.00 293575.00 0.00 254575.00 293575.00 N 4.99 HC IN Daniel B. Asher X1 0.00 293575.00 0.00 254575.00 293575.00 N 4.99 HC IN Intracoastal Capital LLC DE 0.00 293575.00 0.00 254575.00 293575.00 N 4.99 OO Amesite Inc. 607 Shelby Street, Suite 700 PMB 214, Detroit, MI 48226 This Schedule 13G is being filed on behalf of (i) Mitchell P. Kopin, an individual ("Mr. Kopin"), (ii) Daniel B. Asher, an individual ("Mr. Asher") and (iii) Intracoastal Capital LLC, a Delaware limited liability company ("Intracoastal" and together with Mr. Kopin and Mr. Asher, collectively the "Reporting Persons"). The principal business office of Mr. Kopin and Intracoastal is 245 Palm Trail, Delray Beach, Florida 33483. The principal business office of Mr. Asher is 1011 Lake Street, Suite 311, Oak Park, Illinois 60301. Mr. Kopin is a citizen of the United States of America. Mr. Asher is a citizen of the United States of America. Intracoastal is a Delaware limited liability company. Y (i) Immediately following the execution of the Securities Purchase Agreement with the Issuer on April 27, 2026 (the "SPA") (as disclosed in the Form 8-K filed by the Issuer with the Securities and Exchange Commission on April 28, 2026), each of the Reporting Persons may have been deemed to have beneficial ownership of 348,433 shares of Common Stock to be issued to Intracoastal at the closing of the transaction contemplated by the SPA, and all such shares of Common Stock represent beneficial ownership of approximately 7.1% of the Common Stock, based on (1) 4,572,713 shares of Common Stock outstanding as of April 24, 2026, as reported by the Issuer, plus (2) 348,433 shares of Common Stock to be issued to Intracoastal at the closing of the transaction contemplated by the SPA. The foregoing excludes (I) 348,433 shares of Common Stock issuable upon exercise of a warrant to be issued to Intracoastal at the closing of the transaction contemplated by the SPA ("Intracoastal Warrant 1") because Intracoastal Warrant 1 contains a blocker provision under which the holder thereof does not have the right to exercise Intracoastal Warrant 1 to the extent (but only to the extent) that such exercise would result in beneficial ownership by the holder thereof, together with the holder's affiliates, and any other persons acting as a group together with the holder or any of the holder's affiliates, of more than 4.99% of the Common Stock, (II) 696,866 shares of Common Stock issuable upon exercise of a second warrant to be issued to Intracoastal at the closing of the transaction contemplated by the SPA ("Intracoastal Warrant 2") because Intracoastal Warrant 2 is not exercisable until the effective date of stockholder approval of the issuance of the shares of Common Stock issuable upon exercise of Intracoastal Warrant 2 (and Intracoastal Warrant 2 also contains a blocker provision under which the holder thereof does not have the right to exercise Intracoastal Warrant 2 to the extent (but only to the extent) that such exercise would result in beneficial ownership by the holder thereof, together with the holder's affiliates, and any other persons acting as a group together with the holder or any of the holder's affiliates, of more than 4.99% of the Common Stock) and (III) 696,866 shares of Common Stock issuable upon exercise of a third warrant to be issued to Intracoastal at the closing of the transaction contemplated by the SPA ("Intracoastal Warrant 3") because Intracoastal Warrant 3 is not exercisable until the effective date of stockholder approval of the issuance of the shares of Common Stock issuable upon exercise of Intracoastal Warrant 3 (and Intracoastal Warrant 3 also contains a blocker provision under which the holder thereof does not have the right to exercise Intracoastal Warrant 3 to the extent (but only to the extent) that such exercise would result in beneficial ownership by the holder thereof, together with the holder's affiliates, and any other persons acting as a group together with the holder or any of the holder's affiliates, of more than 4.99% of the Common Stock). Without such blocker provisions (and assuming each of Intracoastal Warrant 2 and Intracoastal Warrant 3 was currently exercisable), each of the Reporting Persons may have been deemed to have beneficial ownership of 2,090,598 shares of Common Stock. (ii) As of the close of business on May 1, 2026, each of the Reporting Persons may have been deemed to have beneficial ownership of 293,575 shares of Common Stock, which consisted of (i) 98,000 shares of Common Stock held by Intracoastal and (ii) 195,575 shares of Common Stock issuable upon exercise of Intracoastal Warrant 1, and all such shares of Common Stock represent beneficial ownership of approximately 4.99% of the Common Stock, based on (1) 4,572,713 shares of Common Stock outstanding as of April 24, 2026, as reported by the Issuer, plus (2) 696,866 shares of Common Stock issued at the closing of the transaction contemplated by the SPA, (3) 418,118 shares of Common Stock issued at the closing of the transaction contemplated by another Securities Purchase Agreement entered into by the Issuer on April 27, 2026 (as disclosed in the Form 8-K filed by the Issuer with the Securities and Exchange Commission on April 28, 2026) and (4) 195,575 shares of Common Stock issuable upon exercise of Intracoastal Warrant 1. The foregoing excludes (I) 152,858 shares of Common Stock issuable upon exercise of Intracoastal Warrant 1 because Intracoastal Warrant 1 contains a blocker provision under which the holder thereof does not have the right to exercise Intracoastal Warrant 1 to the extent (but only to the extent) that such exercise would result in beneficial ownership by the holder thereof, together with the holder's affiliates, and any other persons acting as a group together with the holder or any of the holder's affiliates, of more than 4.99% of the Common Stock, (II) 696,866 shares of Common Stock issuable upon exercise of Intracoastal Warrant 2 because Intracoastal Warrant 2 is not exercisable until the effective date of stockholder approval of the issuance of the shares of Common Stock issuable upon exercise of Intracoastal Warrant 2 (and Intracoastal Warrant 2 also contains a blocker provision under which the holder thereof does not have the right to exercise Intracoastal Warrant 2 to the extent (but only to the extent) that such exercise would result in beneficial ownership by the holder thereof, together with the holder's affiliates, and any other persons acting as a group together with the holder or any of the holder's affiliates, of more than 4.99% of the Common Stock) and (III) 696,866 shares of Common Stock issuable upon exercise of Intracoastal Warrant 3 because Intracoastal Warrant 3 is not exercisable until the effective date of stockholder approval of the issuance of the shares of Common Stock issuable upon exercise of Intracoastal Warrant 3 (and Intracoastal Warrant 3 also contains a blocker provision under which the holder thereof does not have the right to exercise Intracoastal Warrant 3 to the extent (but only to the extent) that such exercise would result in beneficial ownership by the holder thereof, together with the holder's affiliates, and any other persons acting as a group together with the holder or any of the holder's affiliates, of more than 4.99% of the Common Stock). Without such blocker provisions (and assuming each of Intracoastal Warrant 2 and Intracoastal Warrant 3 was currently exercisable), each of the Reporting Persons may have been deemed to have beneficial ownership of 1,840,165 shares of Common Stock. 4.99 0 293,575 0 254,575 N Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Exhibit 1 - Joint Filing Agreement Mitchell P. Kopin /s/ Mitchell P. Kopin 05/01/2026 Daniel B. Asher /s/ Daniel B. Asher 05/01/2026 Intracoastal Capital LLC /s/ Mitchell P. Kopin 05/01/2026