<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001804492</cik>
        <entityName>MITOSENSE, INC.</entityName>
        <issuerAddress>
            <street1>330 CHESAPEAKE DRIVE</street1>
            <city>GREAT FALLS</city>
            <stateOrCountry>VA</stateOrCountry>
            <stateOrCountryDescription>VIRGINIA</stateOrCountryDescription>
            <zipCode>22066</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>7032987315</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2019</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>VAN</firstName>
                <middleName>D.</middleName>
                <lastName>HIPP, JR.</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>809 NORTH QUAKER LANE</street1>
                <city>ALEXANDRIA</city>
                <stateOrCountry>VA</stateOrCountry>
                <stateOrCountryDescription>VIRGINIA</stateOrCountryDescription>
                <zipCode>22302</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>TIDAL</firstName>
                <middleName>W.</middleName>
                <lastName>MCCOY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>330 CHESAPEAKE DRIVE</street1>
                <city>GREAT FALLS</city>
                <stateOrCountry>VA</stateOrCountry>
                <stateOrCountryDescription>VIRGINIA</stateOrCountryDescription>
                <zipCode>22066</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>ANDREW</firstName>
                <middleName>E.</middleName>
                <lastName>FINN</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>26 LONGMEADOW STREET</street1>
                <city>LONGMEADOW</city>
                <stateOrCountry>MA</stateOrCountry>
                <stateOrCountryDescription>MASSACHUSETTS</stateOrCountryDescription>
                <zipCode>01106</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>BRENT</firstName>
                <middleName>M.</middleName>
                <lastName>SEGAL</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>5 RANDALL STREET</street1>
                <city>PEMBROKE</city>
                <stateOrCountry>MA</stateOrCountry>
                <stateOrCountryDescription>MASSACHUSETTS</stateOrCountryDescription>
                <zipCode>02359</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>XIANPENG</firstName>
                <lastName>JIANG</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>3529 BROOKMEADE DRIVE</street1>
                <city>BATON ROUGE</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>70816</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>CARLA</firstName>
                <middleName>R.</middleName>
                <lastName>YORK</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>41 RD. 967</street1>
                <city>HENAGER</city>
                <stateOrCountry>AL</stateOrCountry>
                <stateOrCountryDescription>ALABAMA</stateOrCountryDescription>
                <zipCode>35978</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>CATHERINE</firstName>
                <lastName>BAUCOM</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>6415 SEVENOAKS AVE.</street1>
                <city>BATON ROUGE</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>70806</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001804492-21-000001</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2020-02-14</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isOtherType>true</isOtherType>
            <descriptionOfOtherType>Convertible Promissory Notes convertible into Equity Securities</descriptionOfOtherType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>5000</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>ANDREW G. BURCH</recipientName>
                <recipientCRDNumber>2739262</recipientCRDNumber>
                <associatedBDName>CAROLINA SECURITIES INC.</associatedBDName>
                <associatedBDCRDNumber>42869</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1506 SANTA LUCIA ST.</street1>
                    <city>WAKE FOREST</city>
                    <stateOrCountry>NC</stateOrCountry>
                    <stateOrCountryDescription>NORTH CAROLINA</stateOrCountryDescription>
                    <zipCode>27587</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>DE</state>
                    <description>DELAWARE</description>
                    <state>HI</state>
                    <description>HAWAII</description>
                    <state>TX</state>
                    <description>TEXAS</description>
                    <state>MA</state>
                    <description>MASSACHUSETTS</description>
                    <state>MD</state>
                    <description>MARYLAND</description>
                    <state>IA</state>
                    <description>IOWA</description>
                    <state>ME</state>
                    <description>MAINE</description>
                    <state>ID</state>
                    <description>IDAHO</description>
                    <state>MI</state>
                    <description>MICHIGAN</description>
                    <state>UT</state>
                    <description>UTAH</description>
                    <state>MN</state>
                    <description>MINNESOTA</description>
                    <state>MO</state>
                    <description>MISSOURI</description>
                    <state>IL</state>
                    <description>ILLINOIS</description>
                    <state>IN</state>
                    <description>INDIANA</description>
                    <state>MS</state>
                    <description>MISSISSIPPI</description>
                    <state>MT</state>
                    <description>MONTANA</description>
                    <state>AK</state>
                    <description>ALASKA</description>
                    <state>AL</state>
                    <description>ALABAMA</description>
                    <state>VA</state>
                    <description>VIRGINIA</description>
                    <state>AR</state>
                    <description>ARKANSAS</description>
                    <state>NC</state>
                    <description>NORTH CAROLINA</description>
                    <state>NE</state>
                    <description>NEBRASKA</description>
                    <state>RI</state>
                    <description>RHODE ISLAND</description>
                    <state>AZ</state>
                    <description>ARIZONA</description>
                    <state>NH</state>
                    <description>NEW HAMPSHIRE</description>
                    <state>NJ</state>
                    <description>NEW JERSEY</description>
                    <state>VT</state>
                    <description>VERMONT</description>
                    <state>NM</state>
                    <description>NEW MEXICO</description>
                    <state>FL</state>
                    <description>FLORIDA</description>
                    <state>NV</state>
                    <description>NEVADA</description>
                    <state>WA</state>
                    <description>WASHINGTON</description>
                    <state>NY</state>
                    <description>NEW YORK</description>
                    <state>SC</state>
                    <description>SOUTH CAROLINA</description>
                    <state>WI</state>
                    <description>WISCONSIN</description>
                    <state>OH</state>
                    <description>OHIO</description>
                    <state>GA</state>
                    <description>GEORGIA</description>
                    <state>OK</state>
                    <description>OKLAHOMA</description>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>WV</state>
                    <description>WEST VIRGINIA</description>
                    <state>WY</state>
                    <description>WYOMING</description>
                    <state>OR</state>
                    <description>OREGON</description>
                    <state>KS</state>
                    <description>KANSAS</description>
                    <state>CO</state>
                    <description>COLORADO</description>
                    <state>KY</state>
                    <description>KENTUCKY</description>
                    <state>CT</state>
                    <description>CONNECTICUT</description>
                    <state>PA</state>
                    <description>PENNSYLVANIA</description>
                    <state>LA</state>
                    <description>LOUISIANA</description>
                    <state>TN</state>
                    <description>TENNESSEE</description>
                    <state>DC</state>
                    <description>DISTRICT OF COLUMBIA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>true</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>5000000</totalOfferingAmount>
            <totalAmountSold>340000</totalAmountSold>
            <totalRemaining>4660000</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>9</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>120000</dollarAmount>
                <isEstimate>true</isEstimate>
            </salesCommissions>
            <findersFees>
                <dollarAmount>120000</dollarAmount>
                <isEstimate>true</isEstimate>
            </findersFees>
            <clarificationOfResponse>THE ABOVE AMOUNTS ARE GOOD FAITH ESTIMATES SUBJECT TO FUTURE CONTINGENCIES.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>MITOSENSE, INC.</issuerName>
                <signatureName>/Tidal W. McCoy/</signatureName>
                <nameOfSigner>TIDAL W. MCCOY</nameOfSigner>
                <signatureTitle>Vice Chairman &amp; Chief Executive Officer</signatureTitle>
                <signatureDate>2021-02-01</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
