<?xml version="1.0" encoding="UTF-8"?>
<!--EDGARfilingagent.com Form 13F Online Workstation (https://form13f.com)-->
<edgarSubmission xsi:schemaLocation="http://www.sec.gov/edgar/thirteenffiler eis_13F_Filer.xsd" xmlns="http://www.sec.gov/edgar/thirteenffiler" xmlns:ns1="http://www.sec.gov/edgar/common" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <schemaVersion>X0202</schemaVersion>
  <headerData>
    <submissionType>13F-HR</submissionType>
    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <flags>
        <confirmingCopyFlag>false</confirmingCopyFlag>
        <returnCopyFlag>true</returnCopyFlag>
        <overrideInternetFlag>false</overrideInternetFlag>
      </flags>
      <filer>
        <credentials>
          <cik>0001803089</cik>
          <ccc>XXXXXXXX</ccc>
        </credentials>
      </filer>
      <periodOfReport>12-31-2023</periodOfReport>
    </filerInfo>
  </headerData>
  <!--Start Form-->
  <formData>
    <coverPage>
      <reportCalendarOrQuarter>12-31-2023</reportCalendarOrQuarter>
      <!--Amendment-->
      <isAmendment>false</isAmendment>
      <!--Institutional Investment Manager-->
      <filingManager>
        <name>American Family Insurance Mutual Holding Co</name>
        <address>
          <ns1:street1>6000 American Parkway</ns1:street1>
          <ns1:city>Madison</ns1:city>
          <ns1:stateOrCountry>WI</ns1:stateOrCountry>
          <ns1:zipCode>53783</ns1:zipCode>
        </address>
      </filingManager>
      <reportType>13F HOLDINGS REPORT</reportType>
      <form13FFileNumber>028-20013</form13FFileNumber>
      <provideInfoForInstruction5>Y</provideInfoForInstruction5>
      <additionalInformation>American Family Insurance Mutual Holding Company (AFIMHC) is the
direct and indirect parent holding company of
certain "institutional investment managers" as defined under Section
13(f) of the Securities Exchange Act of 1934 (the
Act). Solely for purposes of the reporting requirements under Section
13(f) of the Act and the rules promulgated
thereunder, as a result of the corporate relationship between AFIMHC
and certain of its operating subsidiaries, AFIMHC
is deemed to share investment discretion with such subsidiaries with
respect to Section 13(f) securities. Therefore, the
information required by Form 13F is reported herein by AFIMHC on
behalf of all such subsidiaries to the extent required
by the reporting requirements under Section 13(f).
American Family Insurance Mutual Holding Company (AFIMHC) is the
direct and indirect parent holding company of
certain "institutional investment managers" as defined under Section
13(f) of the Securities Exchange Act of 1934 (the
Act). Solely for purposes of the reporting requirements under Section
13(f) of the Act and the rules promulgated
thereunder, as a result of the corporate relationship between AFIMHC
and certain of its operating subsidiaries, AFIMHC
is deemed to share investment discretion with such subsidiaries with
respect to Section 13(f) securities. Therefore, the
information required by Form 13F is reported herein by AFIMHC on
behalf of all such subsidiaries to the extent required
by the reporting requirements under Section 13(f).</additionalInformation>
    </coverPage>
    <!--Signature-->
    <signatureBlock>
      <name>Lauren K. Powell</name>
      <title>Governance &amp; Licensing, Vice President</title>
      <phone>608-249-2111</phone>
      <signature>Lauren K. Powell, Governance &amp; Licensing, Vice President</signature>
      <city>Madison</city>
      <stateOrCountry>WI</stateOrCountry>
      <signatureDate>02-13-2024</signatureDate>
    </signatureBlock>
    <!--Summary Page-->
    <summaryPage>
      <otherIncludedManagersCount>2</otherIncludedManagersCount>
      <tableEntryTotal>9</tableEntryTotal>
      <tableValueTotal>1369448834</tableValueTotal>
      <isConfidentialOmitted>false</isConfidentialOmitted>
      <!--List of Other Included Managers (Combo Report)-->
      <otherManagers2Info>
        <otherManager2>
          <sequenceNumber>1</sequenceNumber>
          <otherManager>
            <cik>0001803213</cik>
            <form13FFileNumber>028-20014</form13FFileNumber>
            <name>AmFam Holdings, Inc.</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>2</sequenceNumber>
          <otherManager>
            <cik>0000351040</cik>
            <form13FFileNumber>028-00997</form13FFileNumber>
            <name>AMERICAN FAMILY MUTUAL INSURANCE COMPANY, S.I.</name>
          </otherManager>
        </otherManager2>
      </otherManagers2Info>
    </summaryPage>
  </formData>
</edgarSubmission>
