<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001798128</cik>
        <entityName>Jonesboro Bancshares, Inc.</entityName>
        <issuerAddress>
            <street1>109 JIMMIE DAVIS BLVD</street1>
            <city>JONESBORO</city>
            <stateOrCountry>LA</stateOrCountry>
            <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
            <zipCode>71251</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>318-259-4411</issuerPhoneNumber>
        <jurisdictionOfInc>LOUISIANA</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2019</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Charles</firstName>
                <lastName>Allen</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Donald</firstName>
                <lastName>Allen</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Grayson</firstName>
                <lastName>Allen</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Richard</firstName>
                <lastName>Allen</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Thurston</firstName>
                <lastName>Allen</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Christine</firstName>
                <lastName>Weeks</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Paul</firstName>
                <lastName>Weeks</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Robert</firstName>
                <lastName>Earle</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Baker</firstName>
                <lastName>Barr</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>109 Jimmie Davis Blvd</street1>
                <city>Jonesboro</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>71251</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Commercial Banking</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>$5,000,001 - $25,000,000</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001798128-21-000001</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2021-06-09</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isDebtType>true</isDebtType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>1</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Performance Trust Capital Partners, LLC</recipientName>
                <recipientCRDNumber>36155</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>500 West Madison, Suite 450</street1>
                    <city>Chicago</city>
                    <stateOrCountry>IL</stateOrCountry>
                    <stateOrCountryDescription>ILLINOIS</stateOrCountryDescription>
                    <zipCode>60661</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>AL</state>
                    <description>ALABAMA</description>
                    <state>AR</state>
                    <description>ARKANSAS</description>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>CT</state>
                    <description>CONNECTICUT</description>
                    <state>GA</state>
                    <description>GEORGIA</description>
                    <state>KS</state>
                    <description>KANSAS</description>
                    <state>LA</state>
                    <description>LOUISIANA</description>
                    <state>MN</state>
                    <description>MINNESOTA</description>
                    <state>PA</state>
                    <description>PENNSYLVANIA</description>
                    <state>SD</state>
                    <description>SOUTH DAKOTA</description>
                    <state>TX</state>
                    <description>TEXAS</description>
                    <state>WA</state>
                    <description>WASHINGTON</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>14150000</totalOfferingAmount>
            <totalAmountSold>14150000</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse>Original offering was for $12,000,000.  The issuer sold an additional $2,150,000 to one purchaser on August 27, 2021.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>14</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>283000</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>Jonesboro Bancshares, Inc.</issuerName>
                <signatureName>Grayson Allen</signatureName>
                <nameOfSigner>Grayson Allen</nameOfSigner>
                <signatureTitle>CFO</signatureTitle>
                <signatureDate>2021-08-30</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
