<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <!-- Generated by Workiva -->
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001792521</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001465740</issuerCik>
      <issuerName>Two Harbors Investment Corp.</issuerName>
      <secFileNumber>001-34506</secFileNumber>
      <issuerAddress>
        <com:street1>1601 Utica Avenue South</com:street1>
        <com:street2>Suite 900</com:street2>
        <com:city>St. Louis Park</com:city>
        <com:stateOrCountry>MN</com:stateOrCountry>
        <com:zipCode>55416</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>612-453-4100</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>William Ross Greenberg</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Officer</relationshipToIssuer>
        <relationshipToIssuer>Director</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Raymond James</name>
        <address>
          <com:street1>660 Newport Center Drive</com:street1>
          <com:street2>Suite 570</com:street2>
          <com:city>Newport Beach</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>92660</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>9663</noOfUnitsSold>
      <aggregateMarketValue>107259</aggregateMarketValue>
      <noOfUnitsOutstanding>103650126</noOfUnitsOutstanding>
      <approxSaleDate>01/14/2025</approxSaleDate>
      <securitiesExchangeName>NYSE</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <acquiredDate>01/13/2025</acquiredDate>
      <natureOfAcquisitionTransaction>Vesting of Restricted Stock Unit Award</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Two Harbors Investment Corp.</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>9663</amountOfSecuritiesAcquired>
      <paymentDate>01/13/2025</paymentDate>
      <natureOfPayment>Vesting of Restricted Stock Unit Award</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>William Ross Greenberg</name>
        <address>
          <com:street1>1601 Utica Avenue South</com:street1>
          <com:street2>Suite 900</com:street2>
          <com:city>St. Louis Park</com:city>
          <com:stateOrCountry>MN</com:stateOrCountry>
          <com:zipCode>55416</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <saleDate>01/08/2025</saleDate>
      <amountOfSecuritiesSold>14039</amountOfSecuritiesSold>
      <grossProceeds>157387.23</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>William Ross Greenberg</name>
        <address>
          <com:street1>1601 Utica Avenue South</com:street1>
          <com:street2>Suite 900</com:street2>
          <com:city>St. Louis Park</com:city>
          <com:stateOrCountry>MN</com:stateOrCountry>
          <com:zipCode>55416</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <saleDate>01/10/2025</saleDate>
      <amountOfSecuritiesSold>13855</amountOfSecuritiesSold>
      <grossProceeds>154232.26</grossProceeds>
    </securitiesSoldInPast3Months>
    <remarks>The reporting person anticipates selling the shares for the purpose of satisfying income tax liabilities, incurred upon the vesting of a restricted stock unit award, pursuant to written instructions previously executed by the reporting person.</remarks>
    <noticeSignature>
      <noticeDate>01/13/2025</noticeDate>
      <planAdoptionDates>
        <planAdoptionDate>01/26/2023</planAdoptionDate>
      </planAdoptionDates>
      <signature>/s/ William Ross Greenberg</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
