<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001791273</cik>
        <entityName>500 LUCHADORES III, L.P.</entityName>
        <issuerAddress>
            <street1>814 MISSION STREET, 6TH FLOOR</street1>
            <city>SAN FRANCISCO</city>
            <stateOrCountry>CA</stateOrCountry>
            <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
            <zipCode>94103</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>650-318-5408</issuerPhoneNumber>
        <jurisdictionOfInc>ONTARIO, CANADA</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Limited Partnership</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2019</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>N/A</firstName>
                <lastName>500 LUCHADORES III, L.L.C.</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>814 MISSION STREET, 6TH FLOOR</street1>
                <city>SAN FRANCISCO</city>
                <stateOrCountry>CA</stateOrCountry>
                <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
                <zipCode>94103</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>GENERAL PARTNER OF THE ISSUER</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>N/A</firstName>
                <lastName>500 STARTUPS FUND OPS, L.L.C.</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>814 MISSION STREET, 6TH FLOOR</street1>
                <city>SAN FRANCISCO</city>
                <stateOrCountry>CA</stateOrCountry>
                <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
                <zipCode>94103</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>MANAGING MEMBER OF THE GENERAL PARTNER OF THE ISSUER</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>SANTIAGO</firstName>
                <lastName>ZAVALA</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>TAJIN 637, COL. LETRAN VALLE</street1>
                <city>DISTRITO FEDERAL</city>
                <stateOrCountry>O5</stateOrCountry>
                <stateOrCountryDescription>MEXICO</stateOrCountryDescription>
                <zipCode>3650</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>MANAGING MEMBER OF THE GENERAL PARTNER OF THE ISSUER</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>RENE</firstName>
                <lastName>LOMELI</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>AV CHAPULTEPEC 246 INTERIOR 415</street1>
                <city>COLONIA ROMA, DISTRITO FEDERAL</city>
                <stateOrCountry>O5</stateOrCountry>
                <stateOrCountryDescription>MEXICO</stateOrCountryDescription>
                <zipCode>6700</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>MANAGING MEMBER OF THE GENERAL PARTNER OF THE ISSUER</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>DIDIER</firstName>
                <lastName>QUIROZ</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>CDA RINCON COAPA 16 BIS</street1>
                <city>RINCONADA COAPA TLALPAN CDMX</city>
                <stateOrCountry>O5</stateOrCountry>
                <stateOrCountryDescription>MEXICO</stateOrCountryDescription>
                <zipCode>14330</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>MANAGING MEMBER OF THE GENERAL PARTNER OF THE ISSUER</relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pooled Investment Fund</industryGroupType>
            <investmentFundInfo>
                <investmentFundType>Venture Capital Fund</investmentFundType>
                <is40Act>false</is40Act>
            </investmentFundInfo>
        </industryGroup>
        <issuerSize>
            <revenueRange>Not Applicable</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06c</item>
            <item>3C</item>
            <item>3C.1</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001791273-19-000001</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2019-11-08</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isPooledInvestmentFundType>true</isPooledInvestmentFundType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>Indefinite</totalOfferingAmount>
            <totalAmountSold>6670842</totalAmountSold>
            <totalRemaining>Indefinite</totalRemaining>
            <clarificationOfResponse>The amounts listed above include securities offered pursuant to this offering
as well as securities of the issuer offered pursuant to an offering exempt from
registration under Regulation S promulgated under the Securities Act of 1933.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>30</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse>THE ISSUER'S GENERAL PARTNER, OR AN AFFILIATE OF THE ISSUER'S GENERAL PARTNER, MAY BE ENTITLED TO RECEIVE A MANAGEMENT FEE IN ACCORDANCE WITH THE ISSUER'S GOVERNING DOCUMENTATION, AS AMENDED FROM TIME TO TIME.</clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>500 LUCHADORES III, L.P.</issuerName>
                <signatureName>/s/ Santiago Zavala</signatureName>
                <nameOfSigner>SANTIAGO ZAVALA</nameOfSigner>
                <signatureTitle>Managing Member of the General Partner of the Issuer</signatureTitle>
                <signatureDate>2020-11-06</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
